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Literature summary extracted from

  • Ireton, G.C.; McDermott, G.; Black, M.E.; Stoddard, B.L.
    The structure of Escherichia coli cytosine deaminase (2002), J. Mol. Biol., 315, 687-697.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.4.1 medicine enzyme is of interest both for antimicrobial drug design and for gene therapy applications against tumors Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.5.4.1 10 mg/ml purified recombinant His-tagged enzyme, vapour phase equilibration against 11-14% PEG 8000, 0.1 M HEPES, pH 7.3-7.7, 0.2 M MgCl2, in hanging drop geometry, flash-cooling in a buffer containing 30% v/v glycerol for cryoprotection, also crystallization of a seleno-methionine derivatized enzyme, X-ray diffraction structure determination and analysis at 1.5 A resolution Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.4.1 Fe2+ bound to the active site, the metal ion coordinates a hydroxyl nucleophile, binding structure, overview Escherichia coli
3.5.4.1 Zn2+ enzyme bound, binding structure, overview Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.4.1 48000
-
 6 * 48000, (alphabeta)8 barrel structure, crystal structure Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.4.1 additional information Escherichia coli enzyme is an important member of the pyrimidine salvage pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.4.1 Escherichia coli P25524
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.4.1 cytosine + H2O = uracil + NH3 substrate binding involves a significant conformational change that sequesters the reaction complex from solvent, active site architecture and substrate binding, catalytic mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.4.1 5-fluorocytosine + H2O low activity Escherichia coli 5-fluorouracil + NH3
-
 ?
3.5.4.1 cytosine + H2O
-
 Escherichia coli uracil + NH3
-
 ?
3.5.4.1 additional information enzyme is an important member of the pyrimidine salvage pathway Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.4.1 hexamer 6 * 48000, (alphabeta)8 barrel structure, crystal structure Escherichia coli
3.5.4.1 More stabilized by a unique domain-swapping interaction between enzyme subunits, the active site is located in the mouth of the enzyme barrel Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.5.4.1 CD
-
 Escherichia coli