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Literature summary extracted from

  • Markoglou, N.; Hsuesh, R.; Wainer, I.W.
    Immobilized enzyme reactors based upon the flavoenzymes monoamine oxidase A and B (2004), J. Chromatogr. B, 804, 295-302.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.4 analysis on-line immobilized enzyme reactors can be used for the on-line screening of substances for MAO-A and substrate/inhibitor properties Homo sapiens
1.4.3.4 analysis on-line immobilized enzyme reactors can be used for the on-line screening of substrances for MAO-A and substrate/inhibitor properties Homo sapiens

General Stability

EC Number General Stability Organism
1.4.3.4 membranes containing human MAO-A can be immobilized in an immobilized artificial stationary phase with retention of enzymatic activity Homo sapiens
1.4.3.4 membranes containing human MAO-B can be immobilized in an immobilized artificial stationary phase with retention of enzymatic activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.4 (1R,2R)-(-)-psi-ephedrine IC50 for soluble enzyme: 5.03 mM, IC50 for immobilized enzyme: 88 mM, monoamine oxidase B; IC50 for soluble enzyme: 5.35 mM, IC50 for immobilized enzyme: 14.86 mM, monoamine oxidase A Homo sapiens
1.4.3.4 (1S,2S)-(+)-psi-ephedrine IC50 for soluble enzyme: 0.88 mM, IC50 for immobilized enzyme: 1.77 mM, monoamine oxidase A; IC50 for soluble enzyme: 10 mM, IC50 for immobilized enzyme: 234 mM, monoamine oxidase B Homo sapiens
1.4.3.4 (R)-amphetamine IC50 for soluble enzyme: 0.0311 mM, IC50 for immobilized enzyme: 0.0991 mM, monoamine oxidase A; IC50 for soluble enzyme: 0.246 mM, IC50 for immobilized enzyme: 4.03 mM, monoamine oxidase B Homo sapiens
1.4.3.4 (R,S)-amphetamine IC50 for soluble enzyme: 0.0031 mM, IC50 for immobilized enzyme: 0.0244 mM, monoamine oxidase A; IC50 for soluble enzyme: 0.0625 mM, IC50 for immobilized enzyme: 3 mM, monoamine oxidase B Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.4 0.2044
-
 kynuramine pH 7.5, 37°C, soluble enzyme, monoamine oxidase A Homo sapiens
1.4.3.4 0.4065
-
 kynuramine pH 7.5, 37°C, immobilized enzyme, monoamine oxidase A Homo sapiens
1.4.3.4 1.045
-
 kynuramine pH 7.5, 37°C, soluble enzyme, monoamine oxidase B Homo sapiens
1.4.3.4 5.123
-
 kynuramine pH 7.5, 37°C, immobilized enzyme, monoamine oxidase B Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.4 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.4 kynuramine + H2O + O2
-
 Homo sapiens 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.4 MAO-A
-
 Homo sapiens
1.4.3.4 MAO-B
-
 Homo sapiens
1.4.3.4 monoamine oxidase A
-
 Homo sapiens
1.4.3.4 monoamine oxidase B
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.4.3.4 0.0244
-
 IC50 for soluble enzyme: 0.0031 mM, IC50 for immobilized enzyme: 0.0244 mM, monoamine oxidase A Homo sapiens (R,S)-amphetamine
1.4.3.4 0.0991
-
 IC50 for soluble enzyme: 0.0311 mM, IC50 for immobilized enzyme: 0.0991 mM, monoamine oxidase A Homo sapiens (R)-amphetamine
1.4.3.4 1.77
-
 IC50 for soluble enzyme: 0.88 mM, IC50 for immobilized enzyme: 1.77 mM, monoamine oxidase A Homo sapiens (1S,2S)-(+)-psi-ephedrine
1.4.3.4 3
-
 IC50 for soluble enzyme: 0.0625 mM, IC50 for immobilized enzyme: 3 mM, monoamine oxidase B Homo sapiens (R,S)-amphetamine
1.4.3.4 4.03
-
 IC50 for soluble enzyme: 0.246 mM, IC50 for immobilized enzyme: 4.03 mM, monoamine oxidase B Homo sapiens (R)-amphetamine
1.4.3.4 14.86
-
 IC50 for soluble enzyme: 5.35 mM, IC50 for immobilized enzyme: 14.86 mM, monoamine oxidase A Homo sapiens (1R,2R)-(-)-psi-ephedrine
1.4.3.4 88
-
 IC50 for soluble enzyme: 5.03 mM, IC50 for immobilized enzyme: 88 mM, monoamine oxidase B Homo sapiens (1R,2R)-(-)-psi-ephedrine
1.4.3.4 234
-
 IC50 for soluble enzyme: 10 mM, IC50 for immobilized enzyme: 234 mM, monoamine oxidase B Homo sapiens (1S,2S)-(+)-psi-ephedrine