Literature summary extracted from

Leyval, D.; Uy, D.; Delaunay, S.; Goergen, J.L.; Engasser, J.M.
Characterisation of the enzyme activities involved in the valine biosynthetic pathway in a valine-producing strain of Corynebacterium glutamicum (2003), J. Biotechnol., 104, 241-252.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.1.1.86	leucine	-	Corynebacterium glutamicum
1.1.1.86	valine	-	Corynebacterium glutamicum

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1.1.86	1.4	-	2-acetolactate	pH 7.3, 30°C	Corynebacterium glutamicum
1.1.1.86	11.2	-	NADPH	pH 7.3, 30°C	Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.86	2-acetolactate + NADPH	Corynebacterium glutamicum	the enzyme is the second of the valine pathway	2,3-dihydroxy-3-methylbutanoate + NADP+ + H+	-	r

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.86	Corynebacterium glutamicum	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.1.1.86	partial	Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.1.1.86	0.15	-	-	Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.86	2-acetolactate + NADPH	the enzyme is the second of the valine pathway	Corynebacterium glutamicum	2,3-dihydroxy-3-methylbutanoate + NADP+ + H+	-	r

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.1.1.86	45	-	-	Corynebacterium glutamicum

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.1.1.86	7.5	-	-	Corynebacterium glutamicum

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.1.1.86	NADP+	-	Corynebacterium glutamicum
1.1.1.86	NADPH	-	Corynebacterium glutamicum

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Release 2023.1 (January 2023)