Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hamada, N.; Kodaira, R.; Nogawa, M.; Shinji, K.; Ito, R.; Amano, Y.; Shimosaka, M.; Kanda, T.; Okazaki, M.
    Role of cellulose-binding domain of exocellulase I from white rot basidiomycete Irpex lacteus (2001), J. Biosci. Bioeng., 91, 359-362.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.74 42000
-
 P-42, SDS-PAGE Irpex lacteus
3.2.1.74 53000
-
 Ex-1, SDS-PAGE Irpex lacteus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.74 Irpex lacteus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.74 proteolytic modification Papain digestion of native Ex-1, core fragment obtaining Irpex lacteus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.74 gel filtration Irpex lacteus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.74 0.0011
-
 P-42, cotton, pH 5, at 30ºC Irpex lacteus
3.2.1.74 0.0012
-
 P-42, avicel, pH 5, at 30ºC Irpex lacteus
3.2.1.74 0.0021
-
 P-42, bacterial cellulose, pH 5, at 30ºC Irpex lacteus
3.2.1.74 0.0142
-
 Ex-1, cotton, pH 5, at 30ºC Irpex lacteus
3.2.1.74 0.0145
-
 Ex-1, bacterial cellulose, pH 5, at 30ºC Irpex lacteus
3.2.1.74 0.0209
-
 Ex-1, avicel, pH 5, at 30ºC Irpex lacteus
3.2.1.74 0.0243
-
 Ex-1, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30ºC Irpex lacteus
3.2.1.74 0.0244
-
 P-42, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30ºC Irpex lacteus
3.2.1.74 0.026
-
 Ex-1, carboxymethyl cellulose, pH 5, at 30ºC Irpex lacteus
3.2.1.74 0.0311
-
 P-42, carboxymethyl cellulose, pH 5, at 30ºC Irpex lacteus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.74 4-methylumbelliferyl-beta-D-lactoside + H2O
-
 Irpex lacteus 4-methylumbelliferone + D-lactose
-
 ?
3.2.1.74 avicel + H2O
-
 Irpex lacteus ?
-
 ?
3.2.1.74 bacterial cellulose + H2O
-
 Irpex lacteus ?
-
 ?
3.2.1.74 carboxymethyl cellulose + H2O
-
 Irpex lacteus ?
-
 ?
3.2.1.74 cotton + H2O
-
 Irpex lacteus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.74 Ex-1 native enzyme Irpex lacteus
3.2.1.74 exocellulase I
-
 Irpex lacteus
3.2.1.74 P-42 core fragment Irpex lacteus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.74 50
-
 Ex-1, carboxymethyl cellulose as substrate Irpex lacteus
3.2.1.74 50
-
 P-42, carboxymethyl cellulose as substrate Irpex lacteus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.74 25 40 P-42, pH 5.0, retains more than 80% of its maximum activity Irpex lacteus
3.2.1.74 25 55 Ex-1, pH 5.0, retains more than 80% of its maximum activity Irpex lacteus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.74 5
-
 Ex-1, carboxymethyl cellulose as substrate Irpex lacteus
3.2.1.74 5
-
 P-42, carboxymethyl cellulose as substrate Irpex lacteus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.74 3 7 Ex-1, retains more than 80% of its maximum activity Irpex lacteus
3.2.1.74 5 6 P-42, retains more than 70% of its maximum activity Irpex lacteus