Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Francis, K.; Russell, B.; Gadda, G.
    Involvement of a flavosemiquinone in the enzymatic oxidation of nitroalkanes catalyzes by 2-nitropropane dioxygenase (2005), J. Biol. Chem., 280, 5195-5204.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.16 expressed in Escherichia coli BL21(DE3) cells Neurospora crassa
1.13.12.16 gene ncd-2, expression in Escherichia coli strain BL21(DE3), subcloning in strain XL-1 Blue Neurospora crassa
1.13.12.16 gene ncd-2, expression in Escherichia coli strain BL21(DE3), subcloning in strain XL-1 Blue; expressed in Escherichia coli BL21(DE3) cells Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.16 additional information
-
 additional information kinetics, steady-state kinetic mechanism Neurospora crassa
1.13.12.16 1.4
-
 1-nitrohexane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 1.4
-
 hexyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 3.1
-
 ethyl nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 5.5
-
 propyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 7.1
-
 1-nitropentane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 10
-
 butyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 15
-
 1-nitrobutane in 50 mM Tris-Cl, pH 8 at 30°C Neurospora crassa
1.13.12.16 17.9
-
 1-nitrobutane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 24
-
 1-Nitropropane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 29
-
 nitroethane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.12.16 Iron stoichiometry of 0.02 FMN per 1 monomer of enzyme Neurospora crassa
1.13.12.16 additional information devoid of iron Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.16 39920
-
 calculated Neurospora crassa
1.13.12.16 39920
-
 calculated from the amino acid composition of the enzyme Neurospora crassa
1.13.12.16 40000
-
 SDS-PAGE Neurospora crassa
1.13.12.16 80000
-
 recombinant enzyme, gel filtration Neurospora crassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.16 additional information Neurospora crassa enzyme is more specific for nitronates than nitroalkanes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.16 Neurospora crassa Q01284
-
-
1.13.12.16 Neurospora crassa Q01284 gene ncd-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.12.16 DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography Neurospora crassa
1.13.12.16 DEAE fast flow column chromatography and Hi-Prep 16/10 octyl fast flow column chromatography; recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography Neurospora crassa
1.13.12.16 recombinant enzyme from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, DEAE and octyl resin chromatography Neurospora crassa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.12.16 13
-
 cell free extract Neurospora crassa
1.13.12.16 49
-
 after DEAE-Sepharose column chromatography purification only Neurospora crassa
1.13.12.16 80
-
 at 25°C assay temperature, purified recombinant enzyme, substrate ethyl nitronate Neurospora crassa
1.13.12.16 80
-
 after DEAE-Sepharose column chromatography and octyl-Sepharose column chromatography purification Neurospora crassa
1.13.12.16 80
-
 after DEAE-Sepharose column chromatography and octyl-Sepharose column chromatography purification; at 25°C assay temperature, purified recombinant enzyme, substrate ethyl nitronate Neurospora crassa

Storage Stability

EC Number Storage Stability Organism
1.13.12.16 -20°C, 50 mM potassium phosphate, pH 7.4, 6 months, no loss of activity Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.16 1-nitrobutane + O2
-
 Neurospora crassa butyraldehyde + HNO2
-
 ?
1.13.12.16 1-nitrobutane + O2 neutral and anionic form Neurospora crassa butyraldehyde + HNO2
-
 ?
1.13.12.16 1-nitrobutane + O2
-
 Neurospora crassa butyraldehyde + nitrite
-
 ?
1.13.12.16 1-nitrohexane + O2
-
 Neurospora crassa hexanaldehyde + HNO2
-
 ?
1.13.12.16 1-nitrohexane + O2 neutral and anionic form Neurospora crassa hexanaldehyde + HNO2
-
 ?
1.13.12.16 1-nitrohexane + O2
-
 Neurospora crassa hexanaldehyde + nitrite
-
 ?
1.13.12.16 1-nitropentane + O2
-
 Neurospora crassa pentanaldehyde + HNO2
-
 ?
1.13.12.16 1-nitropentane + O2 neutral and anionic form Neurospora crassa pentanaldehyde + HNO2
-
 ?
1.13.12.16 1-nitropentane + O2
-
 Neurospora crassa pentanaldehyde + nitrite
-
 ?
1.13.12.16 1-nitropropane + O2
-
 Neurospora crassa propionaldehyde + HNO2
-
 ?
1.13.12.16 1-nitropropane + O2 neutral and anionic form Neurospora crassa propionaldehyde + HNO2
-
 ?
1.13.12.16 1-nitropropane + O2
-
 Neurospora crassa propionaldehyde + nitrite
-
 ?
1.13.12.16 2-nitropropane + O2
-
 Neurospora crassa acetone + HNO2
-
 ?
1.13.12.16 2-nitropropane + O2 neutral and anionic form Neurospora crassa acetone + HNO2
-
 ?
1.13.12.16 2-nitropropane + O2
-
 Neurospora crassa acetone + nitrite
-
 ?
1.13.12.16 butyl-1-nitronate + O2
-
 Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 butyl-1-nitronate + O2 neutral and anionic form Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 ethyl nitronate + O2
-
 Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 ethylnitronate + O2 neutral and anionic form Neurospora crassa acetaldehyde + HNO2
-
 ?
1.13.12.16 hexyl-1-nitronate + O2
-
 Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 hexyl-1-nitronate + O2 neutral and anionic form Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 additional information the reduced enzyme can reduce the substrate under anaerobically conditions, substrate specificity with nitroalkanes and alkyl nitronates, O2 is delivered from air-saturated buffer in the assay reaction, enzyme catalyzes the 2-step oxidative denitrification of nitroalkanes to their corresponding carbonyl compounds and nitrite Neurospora crassa ?
-
 ?
1.13.12.16 additional information enzyme is more specific for nitronates than nitroalkanes Neurospora crassa ?
-
 ?
1.13.12.16 nitroethane + O2
-
 Neurospora crassa ethanal + nitrite
-
 ?
1.13.12.16 nitroethane + O2 neutral and anionic form Neurospora crassa ethanal + nitrite
-
 ?
1.13.12.16 nitroethane + O2 + H2O
-
 Neurospora crassa ethanal + nitrite + H2O2
-
 ?
1.13.12.16 pentyl-1-nitronate + O2
-
 Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 pentyl-1-nitronate + O2 neutral and anionic form Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 propyl-1-nitronate + O2
-
 Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 propyl-1-nitronate + O2 neutral and anionic form Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 propyl-1-nitronate + O2
-
 Neurospora crassa ?
-
 ?
1.13.12.16 propyl-2-nitronate + O2
-
 Neurospora crassa ? + nitrite
-
 ?
1.13.12.16 propyl-2-nitronate + O2 neutral and anionic form Neurospora crassa ? + nitrite
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.16 dimer 2 * 40000, recombinant enzyme, SDS-PAGE Neurospora crassa
1.13.12.16 homodimer 2 * 40000, gel filtration, heat denaturation, and mass spectroscopic analysis Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
1.13.12.16 2-nitropropane dioxygenase
-
 Neurospora crassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.12.16 30
-
-
 Neurospora crassa
1.13.12.16 30
-
 assay at Neurospora crassa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.12.16 additional information
-
 additional information kcat at different conditions, overview, effects of presence of superoxide dimutase or catalase on the turnover rate of the enzyme Neurospora crassa
1.13.12.16 0.82
-
 2-Nitropropane pH 8.0, 30°C Neurospora crassa
1.13.12.16 1.3
-
 1-nitrohexane pH 8.0, 30°C Neurospora crassa
1.13.12.16 1.5
-
 1-nitrohexane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 1.7
-
 1-nitrohexane pH 8.0, 30°C Neurospora crassa
1.13.12.16 3
-
 1-nitropentane pH 8.0, 30°C Neurospora crassa
1.13.12.16 3.5
-
 nitroethane pH 8.0, 30°C Neurospora crassa
1.13.12.16 3.6
-
 1-nitrobutane pH 8.0, 30°C Neurospora crassa
1.13.12.16 4.3
-
 1-nitropentane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 5.9
-
 1-nitrobutane pH 8.0, 30°C Neurospora crassa
1.13.12.16 6.2
-
 1-nitrobutane in 50 mM Tris-Cl, pH 8 at 30°C Neurospora crassa
1.13.12.16 8.3
-
 1-Nitropropane pH 8.0, 30°C Neurospora crassa
1.13.12.16 11
-
 nitroethane pH 8.0, 30°C Neurospora crassa
1.13.12.16 14
-
 nitroethane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 15
-
 propyl-2-nitronate pH 8.0, 30°C Neurospora crassa
1.13.12.16 20
-
 1-Nitropropane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 21
-
 hexyl-1-nitronate pH 8.0, 30°C Neurospora crassa
1.13.12.16 22
-
 hexyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 29
-
 propyl-2-nitronate pH 8.0, 30°C Neurospora crassa
1.13.12.16 35
-
 pentyl-1-nitronate pH 8.0, 30°C Neurospora crassa
1.13.12.16 40
-
 ethyl nitronate pH 8.0, 30°C Neurospora crassa
1.13.12.16 42
-
 propyl-1-nitronate pH 8.0, 30°C Neurospora crassa
1.13.12.16 42
-
 butyl-1-nitronate pH 8.0, 30°C Neurospora crassa
1.13.12.16 51
-
 ethyl nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 55
-
 butyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 56
-
 butyl-1-nitronate pH 8.0, 30°C Neurospora crassa
1.13.12.16 57
-
 ethylnitronate pH 8.0, 30°C Neurospora crassa
1.13.12.16 60
-
 propyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.12.16 8
-
-
 Neurospora crassa
1.13.12.16 8
-
 assay at Neurospora crassa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.13.12.16 6 9.5
-
 Neurospora crassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.13.12.16 FMN dependent on, stoichiometry of 0.84 FMN per 1 monomer of enzyme, noncovalently bound Neurospora crassa
1.13.12.16 FMN contains a mol of non-covalently bound FMN per mol of subunit, contains 0.84 FMN per monomer of enzyme Neurospora crassa
1.13.12.16 FMN dependent on, stoichiometry of 0.84 FMN per 1 monomer of enzyme, noncovalently bound, contains a mol of non-covalently bound FMN per mol of subunit, contains 0.84 FMN per monomer of enzyme Neurospora crassa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.12.16 0.35
-
 1-nitrobutane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 0.48
-
 nitroethane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 0.61
-
 1-nitropentane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 0.83
-
 1-Nitropropane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 1.1
-
 1-nitrohexane in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 1.2
-
 pentyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 5.6
-
 butyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 11.4
-
 propyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 16
-
 hexyl-1-nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa
1.13.12.16 16.5
-
 ethyl nitronate in 50 mM Tris-HCl, pH 8 at 30°C Neurospora crassa