EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.8.4 | expression in Escherichia coli | Solanum tuberosum |
1.3.8.4 | expression of wild-type and mutants in Escherichia coli strain JM105 | Homo sapiens |
1.3.8.5 | expression in Escherichia coli strain XL1Blue | Solanum tuberosum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.3.8.4 | L370M/G374A | site-directed mutagenesis, substrate specificity similar to the wild-type enzyme, reduced activity | Homo sapiens |
1.3.8.4 | L95V/A99V/L103V | site-directed mutagenesis, inactive mutant | Homo sapiens |
1.3.8.4 | L95V/A99V/L103V/L370M/G374A | site-directed mutagenesis, substitutions in the human enzyme mimick the potato isovaleryl-CoA dehydrogenase isozyme 1, which shows major 2-methylbutyryl-CoA dehydrogenase activity and rather belongs to EC 1.3.99.12, the mutant enzymes shows modified substrate specificty and also exhibits highest activity with 2-methylbutanoyl-CoA, molecular modeling of the active site | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.4 | 0.0013 | - |
isovaleryl-CoA | per tetramer, recombinant enzyme, 32°C | Solanum tuberosum | |
1.3.8.5 | 0.001 | - |
2-methylbutanoyl-CoA | recombinant enzyme, 32°C | Solanum tuberosum |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.8.4 | 50000 | - |
4 * 50000, about, recombinant enzyme, SDS-PAGE | Solanum tuberosum |
1.3.8.4 | 146000 | - |
recombinant enzyme, gel filtration | Homo sapiens |
1.3.8.4 | 146000 | - |
recombinant enzyme, gel filtration | Solanum tuberosum |
1.3.8.5 | 50000 | - |
4 * 50000, about, SDS-PAGE | Solanum tuberosum |
1.3.8.5 | 188000 | - |
recombinant enzyme, gel filtration | Solanum tuberosum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.4 | isovaleryl-CoA + electron transfer protein | Homo sapiens | - |
3-methylcrotonoyl-CoA + reduced electron transfer protein | - |
? | |
1.3.8.4 | isovaleryl-CoA + electron transfer protein | Solanum tuberosum | - |
3-methylcrotonyl-CoA + reduced electron transfer protein | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.8.4 | Homo sapiens | - |
- |
- |
1.3.8.4 | Solanum tuberosum | Q9FS87 | isozyme IVD2 | - |
1.3.8.5 | Solanum tuberosum | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.8.4 | partially from tuber, recombinant from Escherichia coli to over 90% purity | Solanum tuberosum |
1.3.8.4 | recombinant from Escherichia coli to over 90% purity | Homo sapiens |
1.3.8.5 | recombinant from Escherichia coli to over 90% purity | Solanum tuberosum |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.3.8.5 | 2-methylbutanoyl-CoA + electron-transfer flavoprotein = (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | the isovaleryl-CoA dehydrogenase 1, i.e. IVD1, EC 1.3.99.10, from Solanum tuberosum shows maximal activity with 2-methylbutyryl-CoA, the binding pockets of 2-methylbutyryl-CoA and isovaleryl-CoA dehydrogenase are nearly identical, the enzyme therefore shall also be classified to EC 1.3.99.12, active site structure, residues Met361, Ala365, Val88, Val92, and Val96 are involved in catalysis | Solanum tuberosum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.8.4 | sprout | - |
Solanum tuberosum | - |
1.3.8.4 | tuber | - |
Solanum tuberosum | - |
1.3.8.5 | sprout | - |
Solanum tuberosum | - |
1.3.8.5 | tuber | - |
Solanum tuberosum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.8.4 | 0.0003 | - |
recombinant mutant L95V/A99V/L103V/L370M/G374A, substrate 2-methylbutanoyl-CoA | Homo sapiens |
1.3.8.4 | 0.0011 | - |
recombinant mutant L370M/G374A, substrate 2-methylbutanoyl-CoA | Homo sapiens |
1.3.8.4 | 0.0043 | - |
recombinant wild-type enzyme, substrate 2-methylbutanoyl-CoA | Homo sapiens |
1.3.8.4 | 0.0114 | - |
recombinant mutant L370M/G374A, substrate butyryl-CoA | Homo sapiens |
1.3.8.4 | 0.0152 | - |
recombinant wild-type enzyme, substrate butyryl-CoA | Homo sapiens |
1.3.8.4 | 0.0239 | - |
recombinant mutant L370M/G374A, substrate valeryl-CoA | Homo sapiens |
1.3.8.4 | 0.0364 | - |
recombinant wild-type enzyme, substrate valeryl-CoA | Homo sapiens |
1.3.8.4 | 0.0371 | - |
recombinant mutant L370M/G374A, substrate isovaleryl-CoA | Homo sapiens |
1.3.8.4 | 0.0754 | - |
recombinant wild-type enzyme, substrate isovaleryl-CoA | Homo sapiens |
1.3.8.5 | additional information | - |
- |
Solanum tuberosum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.8.4 | 2-methylbutanoyl-CoA + acceptor | low activity with the wild-type and mutant L95V/A99V/L103V/L370M/G374A | Homo sapiens | 2-methylbut-2-enoyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | butyryl-CoA + acceptor | - |
Homo sapiens | but-2-enoyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | butyryl-CoA + acceptor | low activity | Solanum tuberosum | but-2-enoyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | hexanoyl-CoA + acceptor | low activity | Solanum tuberosum | hex-2-enoyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | isovaleryl-CoA + electron transfer protein | - |
Homo sapiens | 3-methylcrotonoyl-CoA + reduced electron transfer protein | - |
? | |
1.3.8.4 | isovaleryl-CoA + electron transfer protein | - |
Solanum tuberosum | 3-methylcrotonyl-CoA + reduced electron transfer protein | - |
? | |
1.3.8.4 | isovaleryl-CoA + electron transfer protein | best substrate | Solanum tuberosum | 3-methylcrotonyl-CoA + reduced electron transfer protein | - |
? | |
1.3.8.4 | additional information | substrate specificity of wild-type and mutant enzymes | Homo sapiens | ? | - |
? | |
1.3.8.4 | octanoyl-CoA + acceptor | very low activity | Solanum tuberosum | oct-2-enoyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | valeryl-CoA + acceptor | - |
Homo sapiens | pent-2-enoyl-CoA + reduced acceptor | - |
? | |
1.3.8.4 | valeryl-CoA + acceptor | low activity | Solanum tuberosum | pent-2-enoyl-CoA + reduced acceptor | - |
? | |
1.3.8.5 | 2-methylbutanoyl-CoA + electron transfer flavoprotein | - |
Solanum tuberosum | 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein | - |
? | |
1.3.8.5 | butyryl-CoA + electron-transfer flavoprotein | - |
Solanum tuberosum | but-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | hexanoyl-CoA + electron-transfer flavoprotein | - |
Solanum tuberosum | hex-2-enoyl-CoA + reduced electron-transfer flavoprotein | - |
? | |
1.3.8.5 | additional information | no activity with isovaleryl-CoA | Solanum tuberosum | ? | - |
? | |
1.3.8.5 | octanoyl-CoA + electron-transfer flavoprotein | - |
Solanum tuberosum | oct-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? | |
1.3.8.5 | valeryl-CoA + electron-transfer flavoprotein | - |
Solanum tuberosum | pent-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.8.4 | tetramer | 4 * 50000, about, recombinant enzyme, SDS-PAGE | Solanum tuberosum |
1.3.8.5 | tetramer | 4 * 50000, about, SDS-PAGE | Solanum tuberosum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.8.4 | IVD | - |
Homo sapiens |
1.3.8.4 | IVD | - |
Solanum tuberosum |
1.3.8.4 | SBCAD | - |
Homo sapiens |
1.3.8.4 | short/branched chain acyl-CoA dehydrogenase | - |
Homo sapiens |
1.3.8.5 | 2-methylbutyryl-CoA dehydrogenase | - |
Solanum tuberosum |
1.3.8.5 | 2MBCD | - |
Solanum tuberosum |
1.3.8.5 | SBCAD | - |
Solanum tuberosum |
1.3.8.5 | short/branched chain acyl-CoA dehydrogenase | - |
Solanum tuberosum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.8.4 | 32 | - |
assay at | Solanum tuberosum |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.8.4 | 0.98 | - |
isovaleryl-CoA | per tetramer, recombinant enzyme, 32°C | Solanum tuberosum | |
1.3.8.5 | 1.77 | - |
2-methylbutanoyl-CoA | per enzyme tetramer, recombinant enzyme, 32°C | Solanum tuberosum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.8.4 | electron transfer protein | - |
Homo sapiens | |
1.3.8.4 | electron transfer protein | - |
Solanum tuberosum |