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Literature summary extracted from

  • Goetzman, E.S.; Mohsen, A.W.; Prasad, K.; Vockley, J.
    Convergent evolution of a 2-methylbutyryl-CoA dehydrogenase from isovaleryl-CoA dehydrogenase in Solanum tuberosum (2005), J. Biol. Chem., 280, 4873-4879.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.4 expression in Escherichia coli Solanum tuberosum
1.3.8.4 expression of wild-type and mutants in Escherichia coli strain JM105 Homo sapiens
1.3.8.5 expression in Escherichia coli strain XL1Blue Solanum tuberosum

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.4 L370M/G374A site-directed mutagenesis, substrate specificity similar to the wild-type enzyme, reduced activity Homo sapiens
1.3.8.4 L95V/A99V/L103V site-directed mutagenesis, inactive mutant Homo sapiens
1.3.8.4 L95V/A99V/L103V/L370M/G374A site-directed mutagenesis, substitutions in the human enzyme mimick the potato isovaleryl-CoA dehydrogenase isozyme 1, which shows major 2-methylbutyryl-CoA dehydrogenase activity and rather belongs to EC 1.3.99.12, the mutant enzymes shows modified substrate specificty and also exhibits highest activity with 2-methylbutanoyl-CoA, molecular modeling of the active site Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.4 0.0013
-
isovaleryl-CoA per tetramer, recombinant enzyme, 32°C Solanum tuberosum
1.3.8.5 0.001
-
2-methylbutanoyl-CoA recombinant enzyme, 32°C Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.8.4 50000
-
4 * 50000, about, recombinant enzyme, SDS-PAGE Solanum tuberosum
1.3.8.4 146000
-
recombinant enzyme, gel filtration Homo sapiens
1.3.8.4 146000
-
recombinant enzyme, gel filtration Solanum tuberosum
1.3.8.5 50000
-
4 * 50000, about, SDS-PAGE Solanum tuberosum
1.3.8.5 188000
-
recombinant enzyme, gel filtration Solanum tuberosum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.4 isovaleryl-CoA + electron transfer protein Homo sapiens
-
3-methylcrotonoyl-CoA + reduced electron transfer protein
-
?
1.3.8.4 isovaleryl-CoA + electron transfer protein Solanum tuberosum
-
3-methylcrotonyl-CoA + reduced electron transfer protein
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.4 Homo sapiens
-
-
-
1.3.8.4 Solanum tuberosum Q9FS87 isozyme IVD2
-
1.3.8.5 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.8.4 partially from tuber, recombinant from Escherichia coli to over 90% purity Solanum tuberosum
1.3.8.4 recombinant from Escherichia coli to over 90% purity Homo sapiens
1.3.8.5 recombinant from Escherichia coli to over 90% purity Solanum tuberosum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.8.5 2-methylbutanoyl-CoA + electron-transfer flavoprotein = (E)-2-methylbut-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+ the isovaleryl-CoA dehydrogenase 1, i.e. IVD1, EC 1.3.99.10, from Solanum tuberosum shows maximal activity with 2-methylbutyryl-CoA, the binding pockets of 2-methylbutyryl-CoA and isovaleryl-CoA dehydrogenase are nearly identical, the enzyme therefore shall also be classified to EC 1.3.99.12, active site structure, residues Met361, Ala365, Val88, Val92, and Val96 are involved in catalysis Solanum tuberosum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.8.4 sprout
-
Solanum tuberosum
-
1.3.8.4 tuber
-
Solanum tuberosum
-
1.3.8.5 sprout
-
Solanum tuberosum
-
1.3.8.5 tuber
-
Solanum tuberosum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.8.4 0.0003
-
recombinant mutant L95V/A99V/L103V/L370M/G374A, substrate 2-methylbutanoyl-CoA Homo sapiens
1.3.8.4 0.0011
-
recombinant mutant L370M/G374A, substrate 2-methylbutanoyl-CoA Homo sapiens
1.3.8.4 0.0043
-
recombinant wild-type enzyme, substrate 2-methylbutanoyl-CoA Homo sapiens
1.3.8.4 0.0114
-
recombinant mutant L370M/G374A, substrate butyryl-CoA Homo sapiens
1.3.8.4 0.0152
-
recombinant wild-type enzyme, substrate butyryl-CoA Homo sapiens
1.3.8.4 0.0239
-
recombinant mutant L370M/G374A, substrate valeryl-CoA Homo sapiens
1.3.8.4 0.0364
-
recombinant wild-type enzyme, substrate valeryl-CoA Homo sapiens
1.3.8.4 0.0371
-
recombinant mutant L370M/G374A, substrate isovaleryl-CoA Homo sapiens
1.3.8.4 0.0754
-
recombinant wild-type enzyme, substrate isovaleryl-CoA Homo sapiens
1.3.8.5 additional information
-
-
Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.4 2-methylbutanoyl-CoA + acceptor low activity with the wild-type and mutant L95V/A99V/L103V/L370M/G374A Homo sapiens 2-methylbut-2-enoyl-CoA + reduced acceptor
-
?
1.3.8.4 butyryl-CoA + acceptor
-
Homo sapiens but-2-enoyl-CoA + reduced acceptor
-
?
1.3.8.4 butyryl-CoA + acceptor low activity Solanum tuberosum but-2-enoyl-CoA + reduced acceptor
-
?
1.3.8.4 hexanoyl-CoA + acceptor low activity Solanum tuberosum hex-2-enoyl-CoA + reduced acceptor
-
?
1.3.8.4 isovaleryl-CoA + electron transfer protein
-
Homo sapiens 3-methylcrotonoyl-CoA + reduced electron transfer protein
-
?
1.3.8.4 isovaleryl-CoA + electron transfer protein
-
Solanum tuberosum 3-methylcrotonyl-CoA + reduced electron transfer protein
-
?
1.3.8.4 isovaleryl-CoA + electron transfer protein best substrate Solanum tuberosum 3-methylcrotonyl-CoA + reduced electron transfer protein
-
?
1.3.8.4 additional information substrate specificity of wild-type and mutant enzymes Homo sapiens ?
-
?
1.3.8.4 octanoyl-CoA + acceptor very low activity Solanum tuberosum oct-2-enoyl-CoA + reduced acceptor
-
?
1.3.8.4 valeryl-CoA + acceptor
-
Homo sapiens pent-2-enoyl-CoA + reduced acceptor
-
?
1.3.8.4 valeryl-CoA + acceptor low activity Solanum tuberosum pent-2-enoyl-CoA + reduced acceptor
-
?
1.3.8.5 2-methylbutanoyl-CoA + electron transfer flavoprotein
-
Solanum tuberosum 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
-
?
1.3.8.5 butyryl-CoA + electron-transfer flavoprotein
-
Solanum tuberosum but-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+
-
?
1.3.8.5 hexanoyl-CoA + electron-transfer flavoprotein
-
Solanum tuberosum hex-2-enoyl-CoA + reduced electron-transfer flavoprotein
-
?
1.3.8.5 additional information no activity with isovaleryl-CoA Solanum tuberosum ?
-
?
1.3.8.5 octanoyl-CoA + electron-transfer flavoprotein
-
Solanum tuberosum oct-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+
-
?
1.3.8.5 valeryl-CoA + electron-transfer flavoprotein
-
Solanum tuberosum pent-2-enoyl-CoA + reduced electron-transfer flavoprotein + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.3.8.4 tetramer 4 * 50000, about, recombinant enzyme, SDS-PAGE Solanum tuberosum
1.3.8.5 tetramer 4 * 50000, about, SDS-PAGE Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
1.3.8.4 IVD
-
Homo sapiens
1.3.8.4 IVD
-
Solanum tuberosum
1.3.8.4 SBCAD
-
Homo sapiens
1.3.8.4 short/branched chain acyl-CoA dehydrogenase
-
Homo sapiens
1.3.8.5 2-methylbutyryl-CoA dehydrogenase
-
Solanum tuberosum
1.3.8.5 2MBCD
-
Solanum tuberosum
1.3.8.5 SBCAD
-
Solanum tuberosum
1.3.8.5 short/branched chain acyl-CoA dehydrogenase
-
Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.8.4 32
-
assay at Solanum tuberosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.4 0.98
-
isovaleryl-CoA per tetramer, recombinant enzyme, 32°C Solanum tuberosum
1.3.8.5 1.77
-
2-methylbutanoyl-CoA per enzyme tetramer, recombinant enzyme, 32°C Solanum tuberosum

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.4 electron transfer protein
-
Homo sapiens
1.3.8.4 electron transfer protein
-
Solanum tuberosum