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Literature summary extracted from

  • Garrity, J.D.; Carenbauer, A.L.; Herron, L.R.; Crowder, M.W.
    Metal binding Asp-120 in metallo-beta-lactamase L1 from Stenotrophomonas maltophilia plays a crucial role in catalysis (2004), J. Biol. Chem., 279, 920-927.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expression of wild-type and mutant enzymes in Escherichia coli Stenotrophomonas maltophilia

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 D120C site-directed mutagenesis, reduced activity and metal binding compared to the wild-type enzyme Stenotrophomonas maltophilia
3.5.2.6 D120N site-directed mutagenesis, reduced activity and metal binding compared to the wild-type enzyme Stenotrophomonas maltophilia
3.5.2.6 D120S site-directed mutagenesis, reduced activity and metal binding compared to the wild-type enzyme Stenotrophomonas maltophilia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.0011
-
 cefoxitin wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.0041
-
 nitrocefin wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.0089
-
 cephalothin wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.013
-
 cefaclor wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.015
-
 meropenem wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.032
-
 biapenem wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.033
-
 cephalothin mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.038
-
 penicillin G wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.045
-
 biapenem mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.046
-
 cephalothin mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.053
-
 cefoxitin mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.055
-
 cefoxitin mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.055
-
 ampicillin wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.057
-
 Imipenem wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.06
-
 meropenem mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.065
-
 Imipenem mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.068
-
 nitrocefin mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.069
-
 nitrocefin mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.07
-
 meropenem mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.08
-
 cefaclor mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.081
-
 biapenem mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.094
-
 Imipenem mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.097
-
 meropenem mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.098
-
 cefaclor mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.098
-
 cefoxitin mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.103
-
 nitrocefin mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.128
-
 biapenem mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.15
-
 cephalothin mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.31
-
 Imipenem mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.384
-
 penicillin G mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.55
-
 cefaclor mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 1.33
-
 ampicillin mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.2.6 Zn2+ absolutely required, dinuclear zinc-metalloenzyme, metal-binding Asp, the wild-type conatins 1.9 mol of Zn2+ per mol of subunit, D120 mutant enzymes show reduced metal content, overview Stenotrophomonas maltophilia

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Stenotrophomonas maltophilia P52700 metallo-beta-lactamase L1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6 recombinant wild-type and mutant enzymes from Escherichia coli Stenotrophomonas maltophilia

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.2.6 a beta-lactam + H2O = a substituted beta-amino acid active site, Asp120 is important for catalysis, as well as substrate and Zn2+ binding Stenotrophomonas maltophilia
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Stenotrophomonas maltophilia (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 biapenem + H2O
-
 Stenotrophomonas maltophilia (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-[(6,7-dihydro-5H-pyrazolo[1,2-a][1,2,4]triazol-4-ium-6-yl)sulfanyl]-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylate
-
 ?
3.5.2.6 cefaclor + H2O
-
 Stenotrophomonas maltophilia (2R)-2-[(R)-{[(2R)-2-amino-2-phenylacetyl]amino}(carboxy)methyl]-5-chloro-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cefoxitin + H2O
-
 Stenotrophomonas maltophilia (2R)-5-[(carbamoyloxy)methyl]-2-[(S)-carboxy(methoxy)[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cephalotin + H2O
-
 Stenotrophomonas maltophilia (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 imipenem + H2O
-
 Stenotrophomonas maltophilia (5R)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-({2-[(iminomethyl)amino]ethyl}sulfanyl)-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 meropenem + H2O
-
 Stenotrophomonas maltophilia (4R,5S)-5-[(1S,2R)-1-carboxy-2-hydroxypropyl]-3-{[(3S,5S)-5-(dimethylcarbamoyl)pyrrolidin-3-yl]sulfanyl}-4-methyl-4,5-dihydro-1H-pyrrole-2-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Stenotrophomonas maltophilia (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 penicillin G + H2O
-
 Stenotrophomonas maltophilia (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 L1
-
 Stenotrophomonas maltophilia

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.6 25
-
 assay at Stenotrophomonas maltophilia

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.01
-
 penicillin G below, mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.01
-
 ampicillin below, mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.01
-
 penicillin G below, mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.01
-
 ampicillin below, mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.028
-
 nitrocefin mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.18
-
 nitrocefin mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.28
-
 cefaclor mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.29
-
 meropenem mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.3
-
 biapenem mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.3
-
 cefaclor mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.31
-
 cephalothin mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.34
-
 cefoxitin mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.36
-
 Imipenem mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.37
-
 cephalothin mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.5
-
 biapenem mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.56
-
 cefoxitin mutant D120C, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.63
-
 Imipenem mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 0.75
-
 meropenem mutant D120S, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 1.53
-
 cefoxitin mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 1.9
-
 cefoxitin wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 2.54
-
 nitrocefin mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 3.6
-
 biapenem mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 6.3
-
 cefaclor mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 8.1
-
 cephalothin mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 10.6
-
 meropenem mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 15
-
 Imipenem mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 41
-
 nitrocefin wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 42
-
 cefaclor wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 45
-
 penicillin G mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 77
-
 ampicillin mutant D120N, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 82
-
 cephalothin wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 134
-
 biapenem wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 157
-
 meropenem wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 370
-
 Imipenem wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 520
-
 ampicillin wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia
3.5.2.6 600
-
 penicillin G wild-type enzyme, pH 7.0, 25°C Stenotrophomonas maltophilia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.6 7
-
 assay at Stenotrophomonas maltophilia