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Literature summary extracted from

  • Gabrielsen, M.; Bond, C.S.; Hallyburton, I.; Hecht, S.; Bacher, A.; Eisenreich, W.; Rohdich, F.; Hunter, W.N.
    Hexameric assembly of the bifunctional methylerythritol 2,4-cyclodiphosphate synthase and protein-protein associations in the deoxy-xylulose-dependent pathway of isoprenoid precursor biosynthesis (2004), J. Biol. Chem., 279, 52753-52761.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.60
-
 Agrobacterium tumefaciens
2.7.7.60
-
 Campylobacter jejuni
2.7.7.60
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.60 hanging drop vapor diffusion method Agrobacterium tumefaciens
2.7.7.60 hanging drop vapor diffusion method Campylobacter jejuni
2.7.7.60 hanging drop vapor diffusion method Escherichia coli
4.6.1.12 hanging drop vapor diffusion method, crystal structure of IspDF, a bifunctional methylerythritol 4-phosphate cytidyltransferase methylerythritol 2,4-cyclodidiphosphate synthase Campylobacter jejuni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.60 41700
-
 6 * 41700, crystal structure analysis Campylobacter jejuni
2.7.7.60 250000
-
 analytical ultracentrifugation Campylobacter jejuni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate Escherichia coli essential enzyme in he mevalonate-independent pathway of isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate Agrobacterium tumefaciens essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate Campylobacter jejuni essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.60 Agrobacterium tumefaciens
-
-
-
2.7.7.60 Campylobacter jejuni
-
-
-
2.7.7.60 Escherichia coli Q46893
-
-
4.6.1.12 Agrobacterium tumefaciens
-
-
-
4.6.1.12 Campylobacter jejuni
-
-
-
4.6.1.12 Escherichia coli P62617
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.60
-
 Agrobacterium tumefaciens
2.7.7.60
-
 Campylobacter jejuni
2.7.7.60
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Agrobacterium tumefaciens diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Campylobacter jejuni diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate
-
 Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate essential enzyme in he mevalonate-independent pathway of isoprenoid biosynthesis Escherichia coli diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis Agrobacterium tumefaciens diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?
2.7.7.60 CTP + 2-C-methyl-D-erythritol 4-phosphate essential enzyme in the mevalonate-independent pathway of isoprenoid biosynthesis Campylobacter jejuni diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.60 hexamer
-
 Agrobacterium tumefaciens
2.7.7.60 hexamer 6 * 41700, crystal structure analysis Campylobacter jejuni
4.6.1.12 hexamer complex formation of IspDF with 4-diphosphocytidyl-2C-methyl-D-erythritol kinase is observed in solution Campylobacter jejuni
4.6.1.12 More complex formation of IspDF with 4-diphosphocytidyl-2C-methyl-D-erythritol kinase is observed in solution Agrobacterium tumefaciens
4.6.1.12 More the monofunctional enzyme 2C-methyl-D-erythritol-4-phosphate cytidyltransferase and 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase shows physical association Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.7.7.60 IspD
-
 Escherichia coli
2.7.7.60 IspDF bifunctional enzyme catalyzing non-consecutive reactions in the 1-deoxy-D-xylulose 5-phosphate pathway of isoprenoid precursor biosynthesis Agrobacterium tumefaciens
2.7.7.60 IspDF bifunctional enzyme catalyzing non-consecutive reactions in the 1-deoxy-D-xylulose 5-phosphate pathway of isoprenoid precursor biosynthesis Campylobacter jejuni
4.6.1.12 IspDF bifunctional methylerythritol 4-phosphate cytidyltransferase methylerythritol 2,4-cyclodidiphosphate synthase. Complex formation of IspDF with 4-diphosphocytidyl-2C-methyl-D-erythritol kinase is observed in solution Agrobacterium tumefaciens
4.6.1.12 IspDF bifunctional methylerythritol 4-phosphate cytidyltransferase methylerythritol 2,4-cyclodidiphosphate synthase.Complex formation of IspDF with 4-diphosphocytidyl-2C-methyl-D-erythritol kinase is observed in solution Campylobacter jejuni
4.6.1.12 IspE monofunctional enzyme Escherichia coli