EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.6.1.13 | Zn2+-bound enzyme, binary complex with ADPribose, ternary complex with Zn2+ and ADPribose, ternary complex with Gd3+ and ADPribose, product complex with AMP and Mg2+, product complex with ribose 5'-phosphate and Zn2+, mutant enzyme E82Q with ligands Mg2+ and SO42-, mutant enzyme E86Q with ligands Mg2+ and ADPribose, mutant enzyme E82Q with ligands Zn2+ and SO42-, mutant enzyme E86Q with ligands Zn2+ and ADPribose | Thermus thermophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.13 | D126N | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 40% of the of the wild-type enzyme | Thermus thermophilus |
3.6.1.13 | D128N | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 74% of the of the wild-type enzyme | Thermus thermophilus |
3.6.1.13 | E127Q | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 114% of the of the wild-type enzyme | Thermus thermophilus |
3.6.1.13 | E129Q | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 32% of the of the wild-type enzyme | Thermus thermophilus |
3.6.1.13 | E82Q | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 0.0006% of the of the wild-type enzyme | Thermus thermophilus |
3.6.1.13 | E86Q | the ratio of turnover-number to Km-value with ADPribose as substrate and Mg2+ as activator is 0.0034% of the of the wild-type enzyme | Thermus thermophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.13 | 0.031 | - |
ADPribose | mutant enzyme D126N, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.044 | - |
ADPribose | mutant enzyme E129Q, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.058 | - |
ADPribose | mutant enzyme D128N, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.071 | - |
ADPribose | mutant enzyme E127Q, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.09 | - |
ADPribose | mutant enzyme E86Q, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.11 | - |
ADPribose | wild-type enzyme, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.215 | - |
ADPribose | mutant enzyme E82Q, in presence of Zn2+ | Thermus thermophilus | |
3.6.1.13 | 0.325 | - |
ADPribose | mutant enzyme E86Q, in presence of Zn2+ | Thermus thermophilus | |
3.6.1.13 | 0.36 | - |
ADPribose | mutant enzyme E82Q, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.37 | - |
ADPribose | wild-type enzyme, in presence of Zn2+ | Thermus thermophilus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.13 | ADP-ribose + H2O | Thermus thermophilus | the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation | AMP + D-ribose 5-phosphate | - |
? | |
3.6.1.13 | ADP-ribose + H2O | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation | AMP + D-ribose 5-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.13 | Thermus thermophilus | - |
- |
- |
3.6.1.13 | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.13 | ADP-ribose + H2O | - |
Thermus thermophilus | AMP + D-ribose 5-phosphate | - |
? | |
3.6.1.13 | ADP-ribose + H2O | the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation | Thermus thermophilus | AMP + D-ribose 5-phosphate | - |
? | |
3.6.1.13 | ADP-ribose + H2O | - |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + D-ribose 5-phosphate | - |
? | |
3.6.1.13 | ADP-ribose + H2O | the enzyme plays a key role in regulating the intracellular ADPribose levels, and prevents nonenzymatic ADP-ribosylation | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | AMP + D-ribose 5-phosphate | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.13 | 0.00011 | - |
ADP-ribose | mutant enzyme E82Q, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.00014 | - |
ADP-ribose | mutant enzyme E82Q, in presence of Zn2+ | Thermus thermophilus | |
3.6.1.13 | 0.00015 | - |
ADP-ribose | mutant enzyme E86Q, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.00032 | - |
ADP-ribose | mutant enzyme E86Q, in presence of Zn2+ | Thermus thermophilus | |
3.6.1.13 | 0.7 | - |
ADP-ribose | mutant enzyme D126N, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 0.7 | - |
ADP-ribose | mutant enzyme E129Q, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 2 | 8 | ADP-ribose | wild-type enzyme, in presence of Zn2+ | Thermus thermophilus | |
3.6.1.13 | 2.2 | - |
ADP-ribose | mutant enzyme D128N, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 4.1 | - |
ADP-ribose | mutant enzyme E127Q, in presence of Mg2+ | Thermus thermophilus | |
3.6.1.13 | 5.7 | - |
ADP-ribose | wild-type enzyme, in presence of Mg2+ | Thermus thermophilus |