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Literature summary extracted from

  • Sulzenbacher, G.; Bignon, C.; Nishimura, T.; Tarling, C.A.; Withers, S.G.; Henrissat, B.; Bourne, Y.
    Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis (2004), J. Biol. Chem., 279, 13119-13128.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.51 medicine deficiency in enzyme activity is associated with fucosidosis Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.51 the crystal structure of enzyme is determined at 2.5 A resolution by the MAD method Thermotoga maritima

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.51 E266A the mutant exhibit very low hydrolytic activity with p-nitrophenyl alpha-L-fucoside Thermotoga maritima
3.2.1.51 E66A the mutant retains ability to catalyse the hydrolysis of p-nitrophenyl alpha-L-fucoside and fucosyl fluorid, the Km value is increase dramatically Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.05
-
 p-nitrophenyl alpha-L-fucoside pH 5.0, 37°C, wild-type enzyme Thermotoga maritima
3.2.1.51 0.075
-
 alpha-L-fucosyl fluoride pH 5.0, 25°C, mutant E266A Thermotoga maritima
3.2.1.51 0.15
-
 alpha-L-fucosyl fluoride pH 5.0, 25°C, wild-type enzyme Thermotoga maritima
3.2.1.51 2.1
-
 p-nitrophenyl alpha-L-fucoside pH 5.0, 37°C, mutant E66A Thermotoga maritima
3.2.1.51 2.5
-
 alpha-L-fucosyl fluoride pH 5.0, 25°C, mutant E66A Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 300000
-
 gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Thermotoga maritima
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 alpha-L-fucosyl fluoride + H2O
-
 Thermotoga maritima alpha-L-fucose + fluoride
-
 ?
3.2.1.51 p-nitrophenyl alpha-L-fucoside + H2O
-
 Thermotoga maritima p-nitrophenol + alpha-L-fucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 hexamer hexamer of two trimers Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.51 0.13
-
 alpha-L-fucosyl fluoride pH 5.0, 25°C, mutant E266A Thermotoga maritima
3.2.1.51 1.3
-
 p-nitrophenyl alpha-L-fucoside pH 5.0, 37°C, mutant E66A Thermotoga maritima
3.2.1.51 1.4
-
 alpha-L-fucosyl fluoride pH 5.0, 25°C, mutant E66A Thermotoga maritima
3.2.1.51 7.6
-
 alpha-L-fucosyl fluoride pH 5.0, 25°C, wild-type enzyme Thermotoga maritima
3.2.1.51 14.3
-
 p-nitrophenyl alpha-L-fucoside pH 5.0, 37°C, wild-type enzyme Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 5
-
-
 Thermotoga maritima