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Literature summary extracted from

  • Akhtar, M.S.; Bhakuni, V.
    Streptococcus pneumoniae hyaluronate lyase contains two non-cooperative independent folding/unfolding structural domains: characterization of functional domain and inhibitors of enzyme (2003), J. Biol. Chem., 278, 25509-25516.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.1 pharmacology enzyme is a target for inhibitor design Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.1 overexpression of His-tagged enzyme in Escherichia coli Streptococcus pneumoniae

General Stability

EC Number General Stability Organism
4.2.2.1 the C-terminal domain is less stable against thermal and guanidine hydrochloride denaturation than the N-terminal domain Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.1 Ca2+ slight inhibition by 30% unfolding of the enzyme by ion binding Streptococcus pneumoniae
4.2.2.1 guanidine hydrochloride strong inhibition, unfolding within 1 h Streptococcus pneumoniae
4.2.2.1 guanidine isothiocyanate strong inhibition Streptococcus pneumoniae
4.2.2.1 L-arginine strong inhibition Streptococcus pneumoniae
4.2.2.1 L-arginine methyl ester strong inhibition Streptococcus pneumoniae
4.2.2.1 Mg2+ slight inhibition by 30% unfolding of the enzyme by ion binding Streptococcus pneumoniae
4.2.2.1 additional information no inhibition and conformational change induced by NaCl Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.1 41000
-
 x * 41000, N-terminal domain, SDS-PAGE, x * 41000, C-terminal domain, SDS-PAGE Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.1 hyaluronan Streptococcus pneumoniae
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.1 Streptococcus pneumoniae Q54873
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.1 isolated N-terminal domain from purified recombinant native enzyme Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.1 hyaluronan
-
 Streptococcus pneumoniae ?
-
 ?
4.2.2.1 hyaluronan native enzyme and isolated N-terminal domain Streptococcus pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.1 ? x * 41000, N-terminal domain, SDS-PAGE, x * 41000, C-terminal domain, SDS-PAGE Streptococcus pneumoniae
4.2.2.1 More enzyme consists of 2 structural domains, C-terminal and N-terminal, each of which folds and unfolds independent from the other due to lack of cooperative interactions between them, structure determination Streptococcus pneumoniae

Synonyms

EC Number Synonyms Comment Organism
4.2.2.1 spnHL
-
 Streptococcus pneumoniae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.1 25
-
 assay at Streptococcus pneumoniae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.1 additional information
-
 transition temperatures Streptococcus pneumoniae
4.2.2.1 55
-
 partially unfolding Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.1 6
-
 assay at Streptococcus pneumoniae