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Literature summary extracted from

  • Collins, T.; Meuwis, M.A.; Stals, I.; Claeyssens, M.; Feller, G.; Gerday, C.
    A novel family 8 xylanase, functional and physicochemical characterization (2002), J. Biol. Chem., 277, 35133-35139.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3) Pseudoalteromonas haloplanktis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Cu2+ 16% inhibition at 1 mM Pseudoalteromonas haloplanktis
3.2.1.8 Hg2+ 31% inhibition at 1 mM Pseudoalteromonas haloplanktis
3.2.1.8 Ni2+ 6% inhibition at 1 mM Pseudoalteromonas haloplanktis
3.2.1.8 Zn2+ 14% inhibition at 1 mM Pseudoalteromonas haloplanktis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information
-
 Pseudoalteromonas haloplanktis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.8 extracellular
-
 Pseudoalteromonas haloplanktis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 45982
-
 x * 45982, mass spectrometry Pseudoalteromonas haloplanktis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.8 additional information Pseudoalteromonas haloplanktis unrooted evolutionary tree, overview ?
-
 ?
3.2.1.8 xylan + H2O Pseudoalteromonas haloplanktis major constituent of plant hemicellulose forming the cell walls of hards woods, 15-30%, and softwoods, 7-10% D-xylotriose + xylotetraose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Pseudoalteromonas haloplanktis Q8RJN8 gene xyl; psychrophilic enzyme, antarctic bacterium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 recombinant enzyme from Escherichia coli, native enzyme 11fold by ultrafiltration and ion exchange chromatography Pseudoalteromonas haloplanktis

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.8 (Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m the O-glycoside hydrolase catalyzes the random hydrolysis of internal beta-1,4-D-xylosidic linkages of xylan and xylooligosaccharides of maximal 4 residue chain length, inverse stereoselectivity Pseudoalteromonas haloplanktis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 additional information unrooted evolutionary tree, overview Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.8 additional information enzyme shows no cellulase, chitosanase, and lichenase activity Pseudoalteromonas haloplanktis ?
-
 ?
3.2.1.8 xylan + H2O major constituent of plant hemicellulose forming the cell walls of hards woods, 15-30%, and softwoods, 7-10% Pseudoalteromonas haloplanktis D-xylotriose + xylotetraose + ?
-
 ?
3.2.1.8 xylan + H2O from oat spelt and other sources, xylan substrate specificity overview, inverse stereoselectivity, enzyme possesses a large substrate binding cleft with at least 6 xylose-binding sites Pseudoalteromonas haloplanktis D-xylotriose + xylotetraose + ?
-
 ?
3.2.1.8 xylooligosaccharide + H2O long-chain substrates, high activity Pseudoalteromonas haloplanktis D-xylotriose + xylotetraose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 45982, mass spectrometry Pseudoalteromonas haloplanktis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 More enzyme belongs to family 8 of glycosyl hydrolases Pseudoalteromonas haloplanktis
3.2.1.8 xylanase
-
 Pseudoalteromonas haloplanktis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 additional information
-
 psychrophilic enzyme with high activity at low temperatures and low thermal stability Pseudoalteromonas haloplanktis
3.2.1.8 25
-
 cold-adapted enzyme Pseudoalteromonas haloplanktis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.8 5 35 60% of maximal activity at 5°C Pseudoalteromonas haloplanktis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 additional information
-
 psychrophilic enzyme with high activity at low temperatures and low thermal stability Pseudoalteromonas haloplanktis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.8 1247
-
 xylan pH 6.5, 25°C Pseudoalteromonas haloplanktis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5.3 8 at 25°C Pseudoalteromonas haloplanktis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 6 9
-
 Pseudoalteromonas haloplanktis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Pseudoalteromonas haloplanktis
-
-
 9.5