BRENDA - Enzyme Database

The CWH8 gene encodes a dolichyl pyrophosphate phosphatase with a luminally oriented active site in the endoplasmic reticulum of Saccharomyces cerevisiae

Fernandez, F.; Rush, J.S.; Toke, D.A.; Han, G.S.; Quinn, J.E.; Carman, G.M.; Choi, J.Y.; Voelker, D.R.; Aebi, M.; Waechter, C.J.; J. Biol. Chem. 276, 41455-41464 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.6.1.43
overexpression in Saccharomyces cerevisiae and Sf-9 insect cells
Saccharomyces cerevisiae
General Stability
EC Number
General Stability
Organism
3.6.1.43
the enzyme is labile to Triton X-100 when expressed in Sf-9 cells
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.43
0.04
-
dolichyl diphosphate
pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
Saccharomyces cerevisiae
3.6.1.43
0.048
-
dolichyl phosphate
pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.43
endoplasmic reticulum
transmembrane protein with a luminally oriented active site
Saccharomyces cerevisiae
5783
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.6.1.43
Saccharomyces cerevisiae
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
-
656027
Saccharomyces cerevisiae
dolichyl phosphate + phosphate
-
-
-
?
3.6.1.43
dolichyl phosphate + H2O
-
656027
Saccharomyces cerevisiae
dolichol + phosphate
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.43
6
7
in the presence of octyl glucoside
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.6.1.43
overexpression in Saccharomyces cerevisiae and Sf-9 insect cells
Saccharomyces cerevisiae
General Stability (protein specific)
EC Number
General Stability
Organism
3.6.1.43
the enzyme is labile to Triton X-100 when expressed in Sf-9 cells
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6.1.43
0.04
-
dolichyl diphosphate
pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
Saccharomyces cerevisiae
3.6.1.43
0.048
-
dolichyl phosphate
pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.6.1.43
endoplasmic reticulum
transmembrane protein with a luminally oriented active site
Saccharomyces cerevisiae
5783
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.6.1.43
dolichyl diphosphate + H2O
-
656027
Saccharomyces cerevisiae
dolichyl phosphate + phosphate
-
-
-
?
3.6.1.43
dolichyl phosphate + H2O
-
656027
Saccharomyces cerevisiae
dolichol + phosphate
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.6.1.43
6
7
in the presence of octyl glucoside
Saccharomyces cerevisiae