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Literature summary extracted from

  • Fernandez, F.; Rush, J.S.; Toke, D.A.; Han, G.S.; Quinn, J.E.; Carman, G.M.; Choi, J.Y.; Voelker, D.R.; Aebi, M.; Waechter, C.J.
    The CWH8 gene encodes a dolichyl pyrophosphate phosphatase with a luminally oriented active site in the endoplasmic reticulum of Saccharomyces cerevisiae (2001), J. Biol. Chem., 276, 41455-41464.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.43 overexpression in Saccharomyces cerevisiae and Sf-9 insect cells Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
3.6.1.43 the enzyme is labile to Triton X-100 when expressed in Sf-9 cells Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.43 0.04
-
 dolichyl diphosphate pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme Saccharomyces cerevisiae
3.6.1.43 0.048
-
 dolichyl phosphate pH 7.2, 30°C, using microsomal fractions from Sf-9 cells expressing the enzyme Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.6.1.43 endoplasmic reticulum transmembrane protein with a luminally oriented active site Saccharomyces cerevisiae 5783
-

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.43 Saccharomyces cerevisiae
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.43 dolichyl diphosphate + H2O
-
 Saccharomyces cerevisiae dolichyl phosphate + phosphate
-
 ?
3.6.1.43 dolichyl phosphate + H2O
-
 Saccharomyces cerevisiae dolichol + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.43 Dol-P-P phosphatase
-
 Saccharomyces cerevisiae
3.6.1.43 dolichyl pyrophosphate phosphatase
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.6.1.43 6 7 in the presence of octyl glucoside Saccharomyces cerevisiae