BRENDA - Enzyme Database

Purification of the glutamyl-tRNA reductase from Chlamydomonas reinhardtii involved in delta-aminolevulinic acid formation during chlorophyll biosynthesis

Chen, M.W.; Jahn, D.; O'Neill, G.P.; Soll, D.; J. Biol. Chem. 265, 4058-4063 (1990)

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
130000
-
glycerol gradient sedimentation, gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Chlamydomonas reinhardtii
the enzyme is involved in delta-aminolevulinic acid formation during chlorophyll biosynthesis
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Chlamydomonas reinhardtii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.70
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.70
additional information
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
655961
Chlamydomonas reinhardtii
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the enzyme is involved in delta-aminolevulinic acid formation during chlorophyll biosynthesis
655961
Chlamydomonas reinhardtii
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.70
monomer
1 * 130000, SDS-PAGE
Chlamydomonas reinhardtii
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Chlamydomonas reinhardtii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
NADPH
-
Chlamydomonas reinhardtii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
130000
-
glycerol gradient sedimentation, gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Chlamydomonas reinhardtii
the enzyme is involved in delta-aminolevulinic acid formation during chlorophyll biosynthesis
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
-
Chlamydomonas reinhardtii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.2.1.70
additional information
-
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
655961
Chlamydomonas reinhardtii
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the enzyme is involved in delta-aminolevulinic acid formation during chlorophyll biosynthesis
655961
Chlamydomonas reinhardtii
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.70
monomer
1 * 130000, SDS-PAGE
Chlamydomonas reinhardtii