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Literature summary extracted from

  • Han, B.K.; Moon, J.K.; Ryu, Y.W.; Park, Y.H.; Jo, D.H.
    Purification and characterization of acidic chitinases from gizzards of broiler (Gallus gallus L.) (2000), J. Biochem. Mol. Biol., 33, 326-331.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information KM for GAC1: 1.97 mg/ml and for GAC2: 0.42 mg/ml with chitin as substrate Gallus gallus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 48200
-
 x * 57800, SDS-PAGE, GAC2, x * 48200, SDS-PAGE, GAC1 Gallus gallus
3.2.1.14 57800
-
 x * 57800, SDS-PAGE, GAC2, x * 48200, SDS-PAGE, GAC1 Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 gizzard
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 688000
-
 GAC1 after phenyl-sepharose purification step Gallus gallus
3.2.1.14 756300
-
 GAC2 after phenyl-sepharose purification step Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Gallus gallus ?
-
 ?
3.2.1.14 N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
-
 Gallus gallus N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose the major product for GAC1 is the dimer, GAC2 produced the dimer and trimer in an equal quantity ?
3.2.1.14 N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
-
 Gallus gallus N-acetyl-D-glucosamine + N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose the major product for GAC1 is the dimer, GAC2 produced the dimer and trimer in an equal quantity ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 57800, SDS-PAGE, GAC2, x * 48200, SDS-PAGE, GAC1 Gallus gallus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 GAC1
-
 Gallus gallus
3.2.1.14 GAC2
-
 Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 60
-
-
 Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 5
-
-
 Gallus gallus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Gallus gallus chromatofocusing, GAC1 and GAC2 4.8 4.7