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Literature summary extracted from

  • Arakawa, N.; Igarashi, M.; Kazuoka, T.; Oikawa, T.; Soda, K.
    D-arginase of Arthrobacter sp. KUJ 8602: characterization and its identity with Zn(2+)-guanidinobutyrase (2003), J. Biochem., 133, 33-42.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.7 DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli Arthrobacter sp.
3.5.3.10 DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.7 1,10-phenanthroline inactivation due to removal of bound Zn2+, reversible by Zn2+ and Co2+, and partially by Mn2+ Arthrobacter sp.
3.5.3.7 5-Aminovalerate competitive inhibition Arthrobacter sp.
3.5.3.7 Mercaptoacetate strong mixed-type inhibitor Arthrobacter sp.
3.5.3.7 additional information inhibition by thiol carboxylate, metal binding sequence Arthrobacter sp.
3.5.3.10 1,10-phenanthroline inactivation due to removal of bound Zn2+, reversible by Zn2+ and Co2+, and partially by Mn2+ Arthrobacter sp.
3.5.3.10 5-Aminovalerate competitive inhibition Arthrobacter sp.
3.5.3.10 Mercaptoacetate strong mixed-type inhibitor Arthrobacter sp.
3.5.3.10 additional information inhibition by thiol carboxylate, metal binding sequence Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.7 additional information
-
 additional information
-
 Arthrobacter sp.
3.5.3.10 additional information
-
 additional information
-
 Arthrobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.3.7 soluble
-
 Arthrobacter sp.
-
-
3.5.3.10 soluble
-
 Arthrobacter sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.7 Co2+ can substitute for Zn2+ Arthrobacter sp.
3.5.3.7 Mn2+ can partially substitute for Zn2+ Arthrobacter sp.
3.5.3.7 Zn2+ 1 g-atom bound to 1 mol of subunit, dependent on, removal causes loss of activity Arthrobacter sp.
3.5.3.10 Co2+ can substitute for Zn2+ Arthrobacter sp.
3.5.3.10 Mn2+ can partially substitute for Zn2+ Arthrobacter sp.
3.5.3.10 Zn2+ 1 g-atom bound to 1 mol of subunit, dependent on, removal causes loss of activity Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.7 40000
-
 6 * 40000, SDS-PAGE Arthrobacter sp.
3.5.3.7 232000
-
 gel filtration Arthrobacter sp.
3.5.3.10 40000
-
 6 * 40000, SDS-PAGE Arthrobacter sp.
3.5.3.10 232000
-
 gel filtration Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.7 Arthrobacter sp. Q8KZT5 strain KUJ8602
-
3.5.3.7 Arthrobacter sp. KUJ 8602 Q8KZT5 strain KUJ8602
-
3.5.3.10 Arthrobacter sp. Q8KZT5 strain KUJ8602
-
3.5.3.10 Arthrobacter sp. KUJ 8602 Q8KZT5 strain KUJ8602
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.7 113fold Arthrobacter sp.
3.5.3.10 113fold Arthrobacter sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.3.7 4-guanidinobutanoate + H2O = 4-aminobutanoate + urea enzyme is identical with D-arginase, EC 3.5.3.10, substrate guanidino group binding sequence Arthrobacter sp.
a
3.5.3.10 D-arginine + H2O = D-ornithine + urea enzyme is identical with guanidinobutyrase, EC 3.5.3.7, substrate guanidino group binding sequence Arthrobacter sp.
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.7 culture-condition:L-arginine-grown cell
-
 Arthrobacter sp.
-
3.5.3.7 additional information the cells can use D-arginine as sole carbon and nitrogen source Arthrobacter sp.
-
3.5.3.10 culture-condition:L-arginine-grown cell
-
 Arthrobacter sp.
-
3.5.3.10 additional information the cells can use D-arginine as sole carbon and nitrogen source Arthrobacter sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.7 8.71
-
 purified enzyme Arthrobacter sp.
3.5.3.10 8.71
-
 purified enzyme Arthrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.7 3-guanidinopropionate + H2O
-
 Arthrobacter sp. 3-aminopropanoate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O preferred substrate Arthrobacter sp. 4-Aminobutyrate + urea
-
 ?
3.5.3.7 4-Guanidinobutyrate + H2O preferred substrate Arthrobacter sp. KUJ 8602 4-Aminobutyrate + urea
-
 ?
3.5.3.7 D-arginine + H2O
-
 Arthrobacter sp. D-ornithine + urea
-
 ?
3.5.3.7 L-arginine + H2O
-
 Arthrobacter sp. L-ornithine + urea
-
 ?
3.5.3.10 3-guanidinopropionate + H2O
-
 Arthrobacter sp. 3-aminopropanoate + urea
-
 ?
3.5.3.10 3-guanidinopropionate + H2O
-
 Arthrobacter sp. KUJ 8602 3-aminopropanoate + urea
-
 ?
3.5.3.10 4-Guanidinobutyrate + H2O preferred substrate Arthrobacter sp. 4-Aminobutyrate + urea
-
 ?
3.5.3.10 4-Guanidinobutyrate + H2O preferred substrate Arthrobacter sp. KUJ 8602 4-Aminobutyrate + urea
-
 ?
3.5.3.10 D-arginine + H2O
-
 Arthrobacter sp. D-ornithine + urea
-
 ?
3.5.3.10 D-arginine + H2O
-
 Arthrobacter sp. KUJ 8602 D-ornithine + urea
-
 ?
3.5.3.10 L-arginine + H2O
-
 Arthrobacter sp. L-ornithine + urea
-
 ?
3.5.3.10 L-arginine + H2O
-
 Arthrobacter sp. KUJ 8602 L-ornithine + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.7 hexamer 6 * 40000, SDS-PAGE Arthrobacter sp.
3.5.3.10 hexamer 6 * 40000, SDS-PAGE Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.5.3.7 4-guanidinobutyrate amidinobutyrase
-
 Arthrobacter sp.
3.5.3.7 GBase
-
 Arthrobacter sp.
3.5.3.7 More enzyme is identical with D-arginase, EC 3.5.3.10 Arthrobacter sp.
3.5.3.7 Zn2+-guanidinobutyrase
-
 Arthrobacter sp.
3.5.3.10 More enzyme is identical with guanidinobutyrase, EC 3.5.3.7 Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.7 37
-
 assay at Arthrobacter sp.
3.5.3.10 37
-
 assay at Arthrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.7 9
-
 with substrate 4-guanidinobutyrate Arthrobacter sp.
3.5.3.7 9.5
-
 with substrate D-arginine Arthrobacter sp.
3.5.3.10 9
-
 with substrate 4-guanidinobutyrate Arthrobacter sp.
3.5.3.10 9.5
-
 with substrate D-arginine Arthrobacter sp.