EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.188 | expression in Escherichia coli strain BL21(DE3) as GST-fusion protein | Trypanosoma brucei |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.188 | 10 mg/ml purified recombinant enzyme from 0.1 M HEPES/NaOH, pH 7.5, 2.0 M ammonium sulfate, 5% w/v PEG 400, and 0.5 M NADPH by hanging drop vapour diffusion method, 20°C, 3 weeks, X-ray diffraction structure determination at room temperature and analysis at 2.6 A resolution | Trypanosoma brucei |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.1.188 | cytosol | - |
Trypanosoma brucei | 5829 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.188 | prostaglandin H2 + NADPH + H+ | Trypanosoma brucei | - |
prostaglandin F2alpha + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.188 | Trypanosoma brucei | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.188 | recombinant enzyme from Escherichia coli by GSH affinity chromatography, cleavage of the fusion protein glutathione S-tranferase by thrombin, and DEAE ion exchange chromatography | Trypanosoma brucei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.188 | 9,10-phenanthrenequinone + NADPH | - |
Trypanosoma brucei | ? | - |
? | |
1.1.1.188 | additional information | enzyme does not reduce prostaglandins D2 and E2 | Trypanosoma brucei | ? | - |
? | |
1.1.1.188 | prostaglandin H2 + NADPH + H+ | - |
Trypanosoma brucei | prostaglandin F2alpha + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.188 | More | enzyme belongs to the AKR aldo-keto reductase superfamily | Trypanosoma brucei |
1.1.1.188 | PGF2alpha synthase | - |
Trypanosoma brucei |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.188 | 37 | - |
assay at | Trypanosoma brucei |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.188 | 7 | - |
assay at | Trypanosoma brucei |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.188 | NADPH | dependent on | Trypanosoma brucei |