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Literature summary extracted from

  • Venturi, L.L.; Polizeli Mde, L.; Terenzi, H.F.; Furriel Rdos, P.; Jorge, J.A.
    Extracellular beta-D-glucosidase from Chaetomium thermophilum var. coprophilum: production, purification and some biochemical properties (2002), J. Basic Microbiol., 42, 55-66.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Ag2+ 1 mM, complete inhibition Thermochaetoides thermophila
3.2.1.21 cellobiose competitive vs. p-nitrophenyl-beta-D-glucopyranoside Thermochaetoides thermophila
3.2.1.21 Hg2+ 1 mM, complete inhibition Thermochaetoides thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.76
-
 p-nitrophenyl-beta-D-glucopyranoside
-
 Thermochaetoides thermophila
3.2.1.21 3.13
-
 cellobiose
-
 Thermochaetoides thermophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 extracellular
-
 Thermochaetoides thermophila
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 40000
-
 gel filtration Thermochaetoides thermophila
3.2.1.21 43000
-
 1 * 43000, SDS-PAGE Thermochaetoides thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 cellobiose + H2O Thermochaetoides thermophila
-
 2 beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermochaetoides thermophila
-
 var. coprophilum
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.21 glycoprotein heavily glycosylated Thermochaetoides thermophila

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 acetone precipitation, DEAE-cellulose Thermochaetoides thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 6-bromo-2-naphthyl-beta-D-glucopyranoside + H2O
-
 Thermochaetoides thermophila 6-bromo-2-naphthol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermochaetoides thermophila 2 beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O 0.5% of activity with p-nitrophenyl-beta-D-glucopyranoside Thermochaetoides thermophila 2 beta-D-glucose
-
 ?
3.2.1.21 p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Thermochaetoides thermophila p-nitrophenol + beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 43000, SDS-PAGE Thermochaetoides thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 65
-
-
 Thermochaetoides thermophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 35 80 approx. 50% of maximal activity at 50°C, 10% of maximal activity at 80°C Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 50 60 no loss of activity after 2 h, deglycosylated beta-glucosidase, 50% loss of activity after 18 min Thermochaetoides thermophila
3.2.1.21 70
-
 50% loss of activity afer 75 min Thermochaetoides thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.5
-
-
 Thermochaetoides thermophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 3.5 8 approx. 16% of maximal activity at pH 4.5 and pH 6.5, respectively Thermochaetoides thermophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 2.8
-
 cellobiose
-
 Thermochaetoides thermophila

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Thermochaetoides thermophila isoelectric focusing
-
 7