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Literature summary extracted from

  • Coker, J.A.; Sheridan, P.P.; Loveland-Curtze, J.; Gutshall, K.R.; Auman, A.J.; Brenchley, J.E.
    Biochemical characterization of a beta-galactosidase with a low temperature optimum obtained from an antarctic Arthrobacter isolate (2003), J. Bacteriol., 185, 5473-5482.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expression in Escherichia coli Arthrobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 D-galactal
-
 Arthrobacter sp.
3.2.1.23 D-galactose
-
 Arthrobacter sp.
3.2.1.23 EDTA 100 mM, inactivation after 1.5 h Arthrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.6
-
 o-nitrophenyl beta-D-galactopyranoside at 20°C Arthrobacter sp.
3.2.1.23 0.7
-
 o-nitrophenyl beta-D-galactopyranoside at 5°C and at 10°C Arthrobacter sp.
3.2.1.23 0.8
-
 o-nitrophenyl beta-D-galactopyranoside at 18°C Arthrobacter sp.
3.2.1.23 11.5
-
 lactose at 18°C Arthrobacter sp.
3.2.1.23 19.4
-
 lactose at 5°C Arthrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 K+ slight stimulation Arthrobacter sp.
3.2.1.23 Mg2+ stimulation Arthrobacter sp.
3.2.1.23 Mn2+ slight stimulation Arthrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 112000
-
 4 * 112000 Arthrobacter sp.
3.2.1.23 463000
-
 analytical ultracentrifugation, at low temperatures, irreversible dissociation into inactive monomers Arthrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Arthrobacter sp. Q7WTU5 antarctic isolate
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Arthrobacter sp. D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Arthrobacter sp. o-nitrophenol + beta-D-galactose
-
 ?
3.2.1.23 p-nitrophenyl beta-D-galactopyranoside + H2O
-
 Arthrobacter sp. p-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 tetramer 4 * 112000 Arthrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 BgaS
-
 Arthrobacter sp.
3.2.1.23 H-BgaS His-tagged enzyme form Arthrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 15
-
-
 Arthrobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23
-
 30 0°C: about 50% of maximal activity, 30°C: about 60% of maximal activity Arthrobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 4.7
-
 lactose at 5°C Arthrobacter sp.
3.2.1.23 5.2
-
 lactose at 18°C Arthrobacter sp.
3.2.1.23 43
-
 o-nitrophenyl beta-D-galactopyranoside at 5°C Arthrobacter sp.
3.2.1.23 57.2
-
 o-nitrophenyl beta-D-galactopyranoside at 10°C Arthrobacter sp.
3.2.1.23 100
-
 o-nitrophenyl beta-D-galactopyranoside at 18°C Arthrobacter sp.
3.2.1.23 110.1
-
 o-nitrophenyl beta-D-galactopyranoside at 20°C Arthrobacter sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.23 0.218
-
 D-galactal
-
 Arthrobacter sp.
3.2.1.23 12
-
 D-galactose
-
 Arthrobacter sp.