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Literature summary extracted from

  • Frankenberg, L.; Brugna, M.; Hederstedt, L.
    Enterococcus faecalis heme-dependent catalase (2002), J. Bacteriol., 184, 6351-6356.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.6 hemin 0.002-0.01 mM required for half-maximal to maximal enzyme production Enterococcus faecalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.6 expression as His-tagged enzyme in constructed enzyme-deficient Bacillus subtilis strain BLF03, overexpression of wild-type and His-tagged enzyme in Enterococcus faecalis strain V583 utilizing an Escherichia coli-Enterococcus faecalis-shuttle plasmid Enterococcus faecalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.6 cytoplasm
-
 Enterococcus faecalis 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.6 Fe2+ heme-containing enzyme Enterococcus faecalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 54000
-
 x * 54000, recombinant His-tagged enzyme, SDS-PAGE Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Enterococcus faecalis detoxification enzyme, enzyme protects the cell against high concentrations of peroxide O2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Enterococcus faecalis
-
 gene katA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 recombinant His-tagged and wild-type enzymes from Bacillus subtilis strain BLF03, 195fold, and Enterococcus faecalis strain V583 Enterococcus faecalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 culture condition:heme-grown cell heme in the growth medium is absolutely required for enzyme expression Enterococcus faecalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 additional information
-
-
 Enterococcus faecalis
1.11.1.6 82
-
 purified recombinant His-tagged enzyme expressed in Bacillus subtilis Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Enterococcus faecalis O2 + H2O
-
 ?
1.11.1.6 H2O2 detoxification enzyme, enzyme protects the cell against high concentrations of peroxide Enterococcus faecalis O2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 ? x * 54000, recombinant His-tagged enzyme, SDS-PAGE Enterococcus faecalis

Synonyms

EC Number Synonyms Comment Organism
1.11.1.6 KatA
-
 Enterococcus faecalis
1.11.1.6 More enzyme belongs to the family of monofunctional catalases Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.6 heme dependent on, contains 1 protoheme IX group per KatA 54 kDa polypeptide Enterococcus faecalis