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Literature summary extracted from
- Effect of salivary gland adenocarcinoma cell-derived alpha-N-acetylgalactosaminidase on the bioactivity of macrophage activating factor (2004), Int. J. Oncol., 24, 521-528.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.49 | medicine | the enzyme can be employed as a diagnostic/prognostic tool for patients with salivary gland adenocarcima | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.49 | additional information | - |
additional information | - |
Homo sapiens |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.49 | 48000 | - |
x * 48000, SDS-PAGE | Homo sapiens |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.49 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.49 | enzyme from salivary gland cells | Homo sapiens |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.2.1.49 | alpha-D-GalNAc-(1->3)-beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide + H2O = D-GalNAc + beta-D-GalNAc-(1->3)-alpha-D-Gal-(1->4)-beta-D-Gal-(1->4)-beta-D-Glc-(1<->1)-ceramide | the enzyme exhibits an endo-enzyme activity in addition to an exo-activity | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.49 | carcinoma cell | high levels, salivary gland and gingival | Homo sapiens | - |
| 3.2.1.49 | embryo | - |
Homo sapiens | - |
| 3.2.1.49 | fibroblast | low levels, embryonic lung and gingival | Homo sapiens | - |
| 3.2.1.49 | HEL cell | 299, low levels | Homo sapiens | - |
| 3.2.1.49 | keratinocyte | low levels | Homo sapiens | - |
| 3.2.1.49 | lung | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.49 | 0.0002 | - |
cell extract of gingival keratinocyte | Homo sapiens |
| 3.2.1.49 | 0.0003 | - |
cell extract of gingival fibroblast | Homo sapiens |
| 3.2.1.49 | 0.0004 | - |
cell extract of embryonic lung fibroblast | Homo sapiens |
| 3.2.1.49 | 0.005 | - |
cell extract of gingival carcinoma | Homo sapiens |
| 3.2.1.49 | 0.012 | - |
cell extract of salivary gland carcinoma | Homo sapiens |
| 3.2.1.49 | 2279 | - |
purified fraction from salivary gland carcinoma | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.49 | additional information | the enzyme inactivates the macrophage activing factor produced from Gc protein and this results in reduced phagocytic activity and superoxide-generation capacity of monocytes/macrophages | Homo sapiens | ? | - |
? |  |
| 3.2.1.49 | p-nitrophenyl 2-acetamide-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside + H2O | - |
Homo sapiens | p-nitrophenol + 2-acetamide-2-deoxy-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside | - |
? | |
| 3.2.1.49 | p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Homo sapiens | p-nitrophenol + alpha-D-galactopyranose | - |
? | |
| 3.2.1.49 | p-nitrophenyl N-acetyl-alpha-D-galactosaminide + H2O | - |
Homo sapiens | p-nitrophenol + N-acetyl-alpha-D-galactosamine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.49 | ? | x * 48000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.49 | alpha-NaGalase | - |
Homo sapiens |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.49 | 37 | - |
stable for 6 h in 0.05 sodium citrate buffer, pH 4.2 | Homo sapiens |
| 3.2.1.49 | 40 | - |
40% loss of activity after 30 min | Homo sapiens |
| 3.2.1.49 | 45 | - |
55% loss of activity after 10 min | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.49 | 4 | 6.8 | - |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.49 | 3 | 8 | below pH 3.6 the activity declines rapidly and at pH 3.0 retains about 1% of the orginal activity, above pH 7.5 the activity declines rapidly and at pH 8.0 retains about 4% of the orginal activity | Homo sapiens |
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Release 2023.1 (January 2023)
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