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Literature summary extracted from

  • Merino-Trigo, A.; Rodriguez-Berrocal, F.J.; de Miguel, E.; Paez de la Cadena, M.
    Activity and properties of alpha-L-fucosidase are dependent on the state of enterocytic differentiation of HT-29 colon cancer cells (2002), Int. J. Biochem. Cell Biol., 34, 1291-1303.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.51 medicine the differentiation of HT-29 colon cancer cells can be used as a model to study the alteration of the enzyme alpha-L-fucosidase during the progression of the tumoural processs Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.06
-
 4-methylumbelliferyl alpha-L-fucopyranoside pH 5.0, 37°C, undifferentiated HT-29 cells Homo sapiens
3.2.1.51 0.07
-
 4-methylumbelliferyl alpha-L-fucopyranoside pH 5.0, 37°C, differentiated HT-29 cells Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.51 HT-29 cell cell colorectal cancer cells Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 additional information
-
 variation of activity during differentiation Homo sapiens
3.2.1.51 0.003
-
 non-differentiated HT-29 cells Homo sapiens
3.2.1.51 0.009
-
 differentiated HT-29 cells Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-methylumbelliferyl alpha-L-fucopyranoside + H2O
-
 Homo sapiens 4-methylumbelliferone + alpha-L-fucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 More comparison of elution profile of enzyme from undifferentiated and differentiated HT-29 cells through gel filtration and SDS-PAGE, presence of a 50000 Da protein in both cultures Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 additional information
-
 comparison of heat stability of enzyme from undifferentiated and differentiated HT-29 cells Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 additional information
-
 comparison of pH stability of enzyme from undifferentiated and differentiated HT-29 cells, enzymes from undifferentiated and differentiated HT-29 cells are very stable at neutral and basic pH values Homo sapiens