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Literature summary extracted from
- Purification and characterization of beta-glucosidase from honey bees (Apis mellifera) (2002), Insect Biochem. Mol. Biol., 32, 679-690.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 11.3 | - |
p-nitrophenyl-beta-D-glucopyranoside | 35°C, pH 5.0, beta-glucosidase purified from honey sac | Apis mellifera |  |
| 3.2.1.21 | 12 | - |
p-nitrophenyl-beta-D-glucopyranoside | 35°C, pH 5.0, beta-glucosidase purified from ventriculus | Apis mellifera | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 72000 | - |
x * 72000, SDS-PAGE | Apis mellifera |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Apis mellifera | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | - |
Apis mellifera |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | DEAE-Sepharose, Superose, CM-sepharose, Mono S, hydroxyapatite, Superose 12 | Apis mellifera |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | honey sac | - |
Apis mellifera | - |
| 3.2.1.21 | ventriculus | - |
Apis mellifera | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3.49 | - |
beta-glucosidase purified from honey sac | Apis mellifera |
| 3.2.1.21 | 5.66 | - |
beta-glucosidase purified from ventriculus | Apis mellifera |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Apis mellifera | p-nitrophenol + beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 72000, SDS-PAGE | Apis mellifera |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 20 | 60 | activity slowly increases as a function of temperature | Apis mellifera |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 20 | 50 | no loss of activity after 20 min | Apis mellifera |
| 3.2.1.21 | 55 | - |
approx. 80% loss of activity after 20 min | Apis mellifera |
| 3.2.1.21 | 60 | - |
complete loss of activity after 20 min | Apis mellifera |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
- |
Apis mellifera |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3 | 6 | increase in activity until pH 5.5, sharp drop in activity above pH 6.0 | Apis mellifera |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3.5 | 9.5 | no loss of activity after 20 min at 25°C | Apis mellifera |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.21 | Apis mellifera | isoelectric focusing, different isoenzymes probably due to different degrees of glycosylation | - |
4.5 |
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