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Literature summary extracted from

  • Hamdy, H.S.
    Purification and some important characters of extracellular inulinase of Alternaria alternata (Fr.) Keissler (2002), Indian J. Exp. Biol., 40, 1393-1398.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.80 synthesis microbial production of high fructose syrup, alcohol, acetone and butanol Alternaria alternata

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.80 Co2+ 30 min at 30ÂșC, 10 mM, 70% inhibition; 30 min at 30ÂșC, 1 mM, 17% inhibition; 30 min at 30ÂșC, 5 mM, 39% inhibition Alternaria alternata
3.2.1.80 Cu2+ 30 min at 30ÂșC, 10 mM, 100% inhibition; 30 min at 30ÂșC, 1 mM, 23% inhibition; 30 min at 30ÂșC, 5 mM, 87% inhibition Alternaria alternata
3.2.1.80 EDTA 30 min at 30ÂșC, 10 mM, 10% inhibition; 30 min at 30ÂșC, 5 mM, 9% inhibition Alternaria alternata
3.2.1.80 Fe3+ 30 min at 30ÂșC, 10 mM, 86% inhibition; 30 min at 30ÂșC, 1 mM, 19% inhibition; 30 min at 30ÂșC, 5 mM, 60% inhibition Alternaria alternata
3.2.1.80 Hg2+ 30 min at 30ÂșC, 10 mM, 85% inhibition; 30 min at 30ÂșC, 1 mM, 26% inhibition; 30 min at 30ÂșC, 5 mM, 57% inhibition Alternaria alternata
3.2.1.80 iodoacetate 30 min at 30ÂșC, 10 mM, 87% inhibition; 30 min at 30ÂșC, 1 mM, 38% inhibition; 30 min at 30ÂșC, 5 mM, 57% inhibition Alternaria alternata
3.2.1.80 Mn2+ 30 min at 30ÂșC, 10 mM, 20% inhibition Alternaria alternata
3.2.1.80 Sodium arsenate 30 min at 30ÂșC, 10 mM, 50% inhibition; 30 min at 30ÂșC, 1 mM, 20% inhibition; 30 min at 30ÂșC, 5 mM, 38% inhibition Alternaria alternata
3.2.1.80 sodium arsenite 30 min at 30ÂșC, 10 mM, 58% inhibition; 30 min at 30ÂșC, 1 mM, 28% inhibition; 30 min at 30ÂșC, 5 mM, 41% inhibition Alternaria alternata
3.2.1.80 Zn2+ 30 min at 30ÂșC, 10 mM, 66% inhibition; 30 min at 30ÂșC, 1 mM, 22% inhibition; 30 min at 30ÂșC, 5 mM, 39% inhibition Alternaria alternata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.80 66
-
 inulin at 55ÂșC, pH 4.6 Alternaria alternata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.80 extracellular
-
 Alternaria alternata
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.80 Ba2+ 30 min at 30ÂșC, 1 mM, 118% of the relative activity without metal ions Alternaria alternata
3.2.1.80 Ba2+ 30 min at 30ÂșC, 10 mM, 140% of the relative activity without metal ions Alternaria alternata
3.2.1.80 Ba2+ 30 min at 30ÂșC, 5 mM, 132% of the relative activity without metal ions Alternaria alternata
3.2.1.80 Ca2+ 30 min at 30ÂșC, 1 mM, 118% of the relative activity without metal ions Alternaria alternata
3.2.1.80 Ca2+ 30 min at 30ÂșC, 5 mM, 108% of the relative activity without metal ions Alternaria alternata
3.2.1.80 Mg2+ 30 min at 30ÂșC, 1 mM, 110% of the relative activity without metal ions Alternaria alternata
3.2.1.80 Mn2+ 30 min at 30ÂșC, 1 mM, 109% of the relative activity without metal ions Alternaria alternata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.80 115000
-
 gel filtration Alternaria alternata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.80 inulin + H2O Alternaria alternata
-
 oligofructosides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.80 Alternaria alternata
-
 (Fr.) Keissler
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.80 no modification
-
 Alternaria alternata

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.80 by ammonium sulfate precipitation, Sephadex column and DEAE-cellulose column, 62fold Alternaria alternata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.80 culture filtrate
-
 Alternaria alternata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [”mol/min/mg] Specific Activity Maximum [”mol/min/mg] Comment Organism
3.2.1.80 80700
-
-
 Alternaria alternata

Storage Stability

EC Number Storage Stability Organism
3.2.1.80 at either -15ÂșC or at 4ÂșC, 200 mM acetate buffer, pH 4.6, 2 weeks, 50% loss of activity Alternaria alternata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.80 inulin + H2O
-
 Alternaria alternata oligofructosides
-
 ?
3.2.1.80 raffinose + H2O
-
 Alternaria alternata beta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranose
-
 ?
3.2.1.80 sucrose + H2O
-
 Alternaria alternata beta-D-fructose + alpha-D-glucose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.80 exoinulinase
-
 Alternaria alternata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.80 55
-
 pH 4.6 Alternaria alternata

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.80 50
-
 after 50ÂșC there is a gradual loss in activity, pH 4.6 Alternaria alternata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.80 4.5
-
 at 55ÂșC, enzyme retains only 90% of its activity Alternaria alternata

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.80 4 5 at 55ÂșC Alternaria alternata