EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.2.1 | overexpression of wild-type and mutant enzymes in Escherichia coli | Streptococcus pneumoniae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.1 | additional information | - |
additional information | kinetic mechanism, kinetics, pH 6.0, 22°C | Streptococcus pneumoniae | |
4.2.2.1 | 0.05 | - |
hyaluronan | recombinant mutant N349A, pH 6.0, 22°C | Streptococcus pneumoniae | |
4.2.2.1 | 0.08 | - |
hyaluronan hexasaccharide | recombinant wild-type enzyme, pH 6.0, 22°C | Streptococcus pneumoniae | |
4.2.2.1 | 0.12 | - |
hyaluronan | recombinant wild-type enzyme and mutant Y408F, pH 6.0, 22°C | Streptococcus pneumoniae | |
4.2.2.1 | 0.17 | - |
hyaluronan | recombinant mutantR243V, pH 6.0, 22°C | Streptococcus pneumoniae | |
4.2.2.1 | 0.99 | - |
hyaluronan | recombinant mutants H399A and N580A, pH 6.0, 22°C | Streptococcus pneumoniae |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.2.1 | membrane | cell surface | Streptococcus pneumoniae | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.1 | Ca2+ | - |
Streptococcus pneumoniae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.1 | Streptococcus pneumoniae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.1 | recombinant wild-type and mutant enzymes from Escherichia coli | Streptococcus pneumoniae |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.2.1 | [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate | 5-step reaction mechanism, catalytic residues are Arg349, His399, Tyr40, residues responsible for substrate binding and translocation of the remaining substrate are Arg243and Asn580, enzyme and acitive site strucure | Streptococcus pneumoniae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.2.1 | additional information | - |
- |
Streptococcus pneumoniae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.1 | hyaluronan | progressive mode of action, degradation | Streptococcus pneumoniae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | unsaturated disaccharide unit | ? | |
4.2.2.1 | hyaluronan hexasaccharide | progressive mode of action, degradation | Streptococcus pneumoniae | 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine | unsaturated disaccharide unit | ? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.1 | 22 | - |
assay at | Streptococcus pneumoniae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.1 | 6 | - |
assay at | Streptococcus pneumoniae |