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Literature summary extracted from

  • Pelletier, M.F.; Marcil, A.; Sevigny, G.; Jakob, C.A.; Tessier, D.C.; Chevet, E.; Menard, R.; Bergeron, J.J.M.; Thomas, D.Y.
    The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo (2000), Glycobiology, 10, 815-827.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.207 expressed with a baculovirus expression system in Sf9 cells, expressed in Cos7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.207 D542N D542 required for catalytic activity Homo sapiens
3.2.1.207 D564N D564 required for catalytic activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.207 castanospermine
-
 Homo sapiens
3.2.1.207 deoxynojirimycin competitive, strong inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.207 0.5
-
 p-nitrophenyl-alpha-D-glucopyranoside pH 7.5, 37°C, alpha1-beta complex Homo sapiens
3.2.1.207 0.52
-
 p-nitrophenyl-alpha-D-glucopyranoside pH 7.5, 37°C, alpha2-beta complex Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.207 80000
-
 SDS-PAGE, beta subunit Homo sapiens
3.2.1.207 110000
-
 SDS-PAGE, alpha1 subunit Homo sapiens
3.2.1.207 250000
-
 native PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.207 Glc2Man9GlcNAc2 + H2O Homo sapiens involved in the Calnexin Cycle Man9GlcNAc2 + alpha-D-glucose
-
 ?
3.2.1.207 additional information Homo sapiens involved in early glycoprotein biogenesis, catalyzes the hydrolysis of two alpha-1,3-linked glucose residues present on all Asn-linked precursor oligosaccharides ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.207 Homo sapiens P14314
-
-
3.2.1.207 Homo sapiens P14314 beta subunit
-
3.2.1.207 Homo sapiens Q14697
-
-
3.2.1.207 Homo sapiens Q14697 alpha1 isofrom of the alpha subunit, isoforms are formed by alternative splicing
-
3.2.1.207 Homo sapiens Q14697 alpha2 isoform of the alpha subunit, contains a 66 bp insert, isoforms are formed by alternative splicing
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.207 purification of recombinant protein using His-tag Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.207 HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.207 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + D-glucose
-
 ?
3.2.1.207 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Homo sapiens 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.207 Glc2Man9GlcNAc2 + H2O involved in the Calnexin Cycle Homo sapiens Man9GlcNAc2 + alpha-D-glucose
-
 ?
3.2.1.207 additional information involved in early glycoprotein biogenesis, catalyzes the hydrolysis of two alpha-1,3-linked glucose residues present on all Asn-linked precursor oligosaccharides Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.207 dimer alpha, beta, two different isoforms of the alpha subunit identified, alpha1 is the short and alpha2 the long isoform, both isoforms form dimers with the beta subunit, only dimeric form shows catalytic activity Homo sapiens
3.2.1.207 dimer alpha, beta, two different isoforms of the alpha subunit identified, alpha1 is the short and alpha2 the long isoforms, both isoforms forms dimers with the beta subunit, only dimeric form shows catalytic activity Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.207 0.003
-
 deoxynojirimycin pH 7.5, 37°C Homo sapiens
3.2.1.207 0.015
-
 castanospermine pH 7.5, 37°C Homo sapiens
3.2.1.207 0.017
-
 castanospermine pH 7.5, 37°C Homo sapiens