EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.86 | - |
Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.1.86 | D217E | less than 4% reductoisomerase activity in comparison to wild-type enzyme | Escherichia coli |
1.1.1.86 | D217N | less than 4% reductoisomerase activity in comparison to wild-type enzyme | Escherichia coli |
1.1.1.86 | E213D | 75% reductoisomerase activity in comparison of wild-type enzyme | Escherichia coli |
1.1.1.86 | E213Q | less than 4% reductoisomerase activity in comparison of wild-type enzyme | Escherichia coli |
1.1.1.86 | E221D | less than 4% reductoisomerase activity in comparison to wild-type enzyme | Escherichia coli |
1.1.1.86 | E221Q | less than 4% reductoisomerase activity in comparison to wild-type enzyme | Escherichia coli |
1.1.1.86 | E389D | less than 4% reductoisomerase activity in comparison to wild-type enzyme | Escherichia coli |
1.1.1.86 | E389Q | less than 4% reductoisomerase activity in comparison to wild-type enzyme | Escherichia coli |
1.1.1.86 | E393D | less than 4% reductoisomerase activity in comparison to wild-type enzyme | Escherichia coli |
1.1.1.86 | E393Q | the mutant is insoluble, a soluble form is obtained only after denaturation | Escherichia coli |
1.1.1.86 | H132K | less than 4% reductoisomerase activity in comparison of wild-type enzyme | Escherichia coli |
1.1.1.86 | H132Q | less than 4% reductoisomerase activity in comparison of wild-type enzyme | Escherichia coli |
1.1.1.86 | K155E | less than 4% reductoisomerase activity in comparison of wild-type enzyme | Escherichia coli |
1.1.1.86 | K155Q | less than 4% reductoisomerase activity in comparison of wild-type enzyme | Escherichia coli |
1.1.1.86 | K155R | less than 4% reductoisomerase activity in comparison of wild-type enzyme | Escherichia coli |
1.1.1.86 | S414A | less than 4% reductoisomerase activity in comparison to wild-type enzyme | Escherichia coli |
1.1.1.86 | S414T | less than 4% reductoisomerase activity in comparison to wild-type enzyme | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.86 | 0.00016 | - |
NADPH | pH 8.0, 37°C, mutant E213D | Escherichia coli | |
1.1.1.86 | 0.00253 | - |
NADPH | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 0.0031 | - |
NADPH | pH 8.0, 37°C, mutant H132K | Escherichia coli | |
1.1.1.86 | 0.0048 | - |
NADPH | pH 8.0, 37°C, mutant E393D | Escherichia coli | |
1.1.1.86 | 0.005 | - |
NADPH | pH 8.0, 37°C, mutant D217N | Escherichia coli | |
1.1.1.86 | 0.005 | - |
NADPH | pH 8.0, 37°C, mutant S414T | Escherichia coli | |
1.1.1.86 | 0.0073 | - |
NADPH | pH 8.0, 37°C, mutant K155R | Escherichia coli | |
1.1.1.86 | 0.00804 | - |
NADPH | pH 8.0, 37°C, mutant K155E | Escherichia coli | |
1.1.1.86 | 0.0084 | - |
NADPH | pH 8.0, 37°C, mutant S414A | Escherichia coli | |
1.1.1.86 | 0.0093 | - |
NADPH | pH 8.0, 37°C, mutant K155Q | Escherichia coli | |
1.1.1.86 | 0.02 | - |
NADPH | pH 8.0, 37°C, mutant E221D | Escherichia coli | |
1.1.1.86 | 0.02 | - |
NADPH | pH 8.0, 37°C, mutant E221Q | Escherichia coli | |
1.1.1.86 | 0.023 | - |
NADPH | pH 8.0, 37°C, mutant E389D | Escherichia coli | |
1.1.1.86 | 0.069 | - |
NADPH | pH 8.0, 37°C, mutant H132Q | Escherichia coli | |
1.1.1.86 | 0.08 | - |
NADPH | pH 8.0, 37°C, mutant D217E | Escherichia coli | |
1.1.1.86 | 0.17 | - |
2-ketopantoate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 0.21 | - |
3-hydroxy-2-ketobutyrate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 0.25 | - |
2-acetolactate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 0.27 | - |
3-hydroxy-3-methyl-2-ketobutyrate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 0.334 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant S414A | Escherichia coli | |
1.1.1.86 | 0.356 | - |
2-acetolactate | pH 8.0, 37°C, mutant E221D | Escherichia coli | |
1.1.1.86 | 0.414 | - |
2-acetolactate | pH 8.0, 37°C, mutant S414T | Escherichia coli | |
1.1.1.86 | 0.441 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant E213Q | Escherichia coli | |
1.1.1.86 | 0.588 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant E393Q | Escherichia coli | |
1.1.1.86 | 0.711 | - |
2-acetolactate | pH 8.0, 37°C, mutant S414A | Escherichia coli | |
1.1.1.86 | 0.818 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant H132K | Escherichia coli | |
1.1.1.86 | 0.922 | - |
2-acetolactate | pH 8.0, 37°C, mutant H213D | Escherichia coli | |
1.1.1.86 | 0.929 | - |
2-acetolactate | pH 8.0, 37°C, mutant H132Q | Escherichia coli | |
1.1.1.86 | 1.101 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant S414T | Escherichia coli | |
1.1.1.86 | 1.218 | - |
2-acetolactate | pH 8.0, 37°C, mutant H155R | Escherichia coli | |
1.1.1.86 | 1.37 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant E221D | Escherichia coli | |
1.1.1.86 | 1.54 | - |
pyruvate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 2.028 | - |
2-acetolactate | pH 8.0, 37°C, mutant E289D | Escherichia coli | |
1.1.1.86 | 2.66 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant K155E | Escherichia coli | |
1.1.1.86 | 2.96 | - |
3-hydroxypyruvate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 3.15 | - |
2-ketovalerate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 3.32 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant E393D | Escherichia coli | |
1.1.1.86 | 3.67 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant E213D | Escherichia coli | |
1.1.1.86 | 4.56 | - |
2-Ketobutyrate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 6.91 | - |
2-ketoisovalerate | pH 8.0, 37°C, wild-type enzyme | Escherichia coli | |
1.1.1.86 | 7.43 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant H132Q | Escherichia coli | |
1.1.1.86 | 7.64 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant D217N | Escherichia coli | |
1.1.1.86 | 8.5 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant E389D | Escherichia coli | |
1.1.1.86 | 8.88 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant E389Q | Escherichia coli | |
1.1.1.86 | 13.6 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant K155R | Escherichia coli | |
1.1.1.86 | 15.3 | - |
3-hydroxypyruvate | pH 8.0, 37°C, mutant K155Q | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | Co2+ | the reaction requires a divalent metal ion | Escherichia coli | |
1.1.1.86 | Mg2+ | the reaction requires a divalent metal ion | Escherichia coli | |
1.1.1.86 | Mn2+ | the reaction requires a divalent metal ion | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.86 | additional information | Escherichia coli | the enzyme is involved in the biosynthesis of the branched-chain amino acids | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.86 | Escherichia coli | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.86 | of wild-type and mutants | Escherichia coli |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.1.86 | (2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ | bifunctional enzyme that catalyses two different reactions at a common active site, an isomerization consisting of an alkyl migration, followed by an NADPH-dependent reduction of a-ketoacid | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.86 | additional information | - |
the specific activity with pyruvate is 1% and with 2-ketovalerate, 2-ketopantoate and 2-ketobutyrate is 8% of that of 2-acetolactate, comparison of activities of wild-type and mutant enzymes | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.86 | 2-acetolactate + NADPH + H+ | - |
Escherichia coli | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
1.1.1.86 | 2-ketobutyrate + NADPH + H+ | - |
Escherichia coli | ? | - |
r | |
1.1.1.86 | 2-ketoisovalerate + NADPH + H+ | - |
Escherichia coli | ? | - |
r | |
1.1.1.86 | 2-ketopantoate + NADPH + H+ | - |
Escherichia coli | ? | - |
r | |
1.1.1.86 | 2-ketovalerate + NADPH + H+ | - |
Escherichia coli | ? | - |
r | |
1.1.1.86 | 3-hydroxy-2-ketobutyrate + NADPH + H+ | - |
Escherichia coli | ? | - |
r | |
1.1.1.86 | 3-hydroxy-3-methyl-2-ketobutyrate + NADP+ | - |
Escherichia coli | ? | - |
r | |
1.1.1.86 | 3-hydroxypyruvate + NADPH + H+ | - |
Escherichia coli | ? | - |
r | |
1.1.1.86 | additional information | the enzyme is involved in the biosynthesis of the branched-chain amino acids | Escherichia coli | ? | - |
? | |
1.1.1.86 | pyruvate + NADPH + H+ | - |
Escherichia coli | ? | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.86 | 0.021 | - |
pyruvate | pH 8.0, 37°C | Escherichia coli | |
1.1.1.86 | 0.05 | - |
2-ketovalerate | pH 8.0, 37°C | Escherichia coli | |
1.1.1.86 | 0.167 | - |
2-Ketobutyrate | pH 8.0, 37°C | Escherichia coli | |
1.1.1.86 | 0.182 | - |
2-ketoisovalerate | pH 8.0, 37°C | Escherichia coli | |
1.1.1.86 | 0.194 | - |
2-ketopantoate | pH 8.0, 37°C | Escherichia coli | |
1.1.1.86 | 0.594 | - |
3-hydroxy-2-ketobutyrate | pH 8.0, 37°C | Escherichia coli | |
1.1.1.86 | 2.231 | - |
2-acetolactate | pH 8.0, 37°C | Escherichia coli | |
1.1.1.86 | 3.511 | - |
3-hydroxy-3-methyl-2-ketobutyrate | pH 8.0, 37°C | Escherichia coli | |
1.1.1.86 | 5.376 | - |
3-hydroxypyruvate | pH 8.0, 37°C | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.86 | NADP+ | - |
Escherichia coli | |
1.1.1.86 | NADPH | - |
Escherichia coli |