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Literature summary extracted from

  • Chen, S.; Ge, W.; Buswell, J.A.
    Biochemical and molecular characterization of a laccase from the edible straw mushroom, Volvariella volvacea (2004), Eur. J. Biochem., 271, 318-328.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2
-
 Volvariella volvacea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 azide 0.1 mM, 100% inhibition Volvariella volvacea
1.10.3.2 cysteine 0.1 mM, 100% inhibition Volvariella volvacea
1.10.3.2 dithiothreitol 0.1 mM, 100% inhibition Volvariella volvacea
1.10.3.2 EDTA 1 mM, 20% inhibition Volvariella volvacea
1.10.3.2 thioglycolic acid 1 mM, 100% inhibition Volvariella volvacea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.01
-
 syringaldazine pH 5.6, 45°C Volvariella volvacea
1.10.3.2 0.03
-
 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, 45°C Volvariella volvacea
1.10.3.2 0.57
-
 2,6-dimethoxyphenol pH 4.6, 45°C Volvariella volvacea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 58000
-
 x * 58000, SDS-PAGE Volvariella volvacea

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Volvariella volvacea
-
 growth medium supplemented with copper
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Volvariella volvacea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 culture supernatant
-
 Volvariella volvacea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 2.8
-
 substrate 2,6-dimethoxyphenol, pH 4.6 Volvariella volvacea
1.10.3.2 3.4
-
 substrate syringaldazine, pH 5.6 Volvariella volvacea
1.10.3.2 13.5
-
 substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.0 Volvariella volvacea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 Volvariella volvacea ? + H2O
-
 ?
1.10.3.2 2,6-dimethoxyphenol + H2O about 5% of the activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Volvariella volvacea ?
-
 ?
1.10.3.2 additional information poor substrates: guaiacol, catechol, no substrate: tyrosine, L-3,4-dimethoxyphenol, dihydroxyphenylalanine Volvariella volvacea ?
-
 ?
1.10.3.2 syringaldazine + O2 about 17% of the activity with 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Volvariella volvacea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 58000, SDS-PAGE Volvariella volvacea
1.10.3.2 More N-terminal amino acid sequence Volvariella volvacea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 45
-
 substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Volvariella volvacea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 substrate 2,2’-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) Volvariella volvacea
1.10.3.2 4.6
-
 substrate 2,6-dimethoxyphenol Volvariella volvacea
1.10.3.2 5.6
-
 substrate syringaldazine Volvariella volvacea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Volvariella volvacea isoelectric focusing
-
 4.2