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Literature summary extracted from

  • McGee, D.J.; Zabaleta, J.; Viator, R.J.; Testerman, T.L.; Ochoa, A.C.; Mendz, G.L.
    Purification and characterization of Helicobacter pylori arginase, RocF: unique features among the arginase superfamily (2004), Eur. J. Biochem., 271, 1952-1962.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.3.1 additional information activation at 50-55°C in presence of Co2+ Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.1 gene rocF, functional expression in Escherichia coli DH5alpha as His-tagged enzyme Helicobacter pylori

General Stability

EC Number General Stability Organism
3.5.3.1 dialysis results in in loss of over 90% activity, highly unstable enzyme Helicobacter pylori
3.5.3.1 recombinant protein, highly unstable Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.3.1 2-mercaptoethanol 50% inhibition at 0.8 mM Helicobacter pylori
3.5.3.1 dithiothreitol 50% inhibition at 0.025 mM Helicobacter pylori
3.5.3.1 DTT 50% inhibition at 0.025 mM Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.1 21.8
-
 L-arginine pH 7.1, 37°C Helicobacter pylori
3.5.3.1 21.8
-
 L-arginine pH 7.1, 37°C, recombinant enzyme Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.1 Co2+ required Helicobacter pylori
3.5.3.1 Co2+ required, activates, best metal cofactor Helicobacter pylori
3.5.3.1 Mn2+ activates slightly, 14.6% of the activity with Co2+ Helicobacter pylori
3.5.3.1 Mn2+ 15-20% of the activity with Co2+ Helicobacter pylori
3.5.3.1 additional information metalloenzyme, a metal ion is absolutely required for activity, no activation with Zn2+, Cu2+, Fe2+, Ca2+, and Mg2+ Helicobacter pylori
3.5.3.1 Ni2+ activates slightly, 6.1% of the activity with Co2+ Helicobacter pylori
3.5.3.1 Ni2+ 6-30% of the activity with Co2+ Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.3.1 40000
-
 x * 40000, SDS-PAGE, recombinant enzyme with His-tag Helicobacter pylori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.1 L-arginine + H2O Helicobacter pylori enzyme is involved in acid resistance and inhibits host nitric oxide production L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O Helicobacter pylori ATCC 43504 enzyme is involved in acid resistance and inhibits host nitric oxide production L-ornithine + urea
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.1 Helicobacter pylori
-
-
-
3.5.3.1 Helicobacter pylori
-
 gene rocF
-
3.5.3.1 Helicobacter pylori ATCC 43504
-
 gene rocF
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.3.1 recombinant enzyme from Escherichia coli Helicobacter pylori

Storage Stability

EC Number Storage Stability Organism
3.5.3.1 -20°C, His-tagged recombinant protein, 1:2 dilution with 100% glycerol, 4 months, 45-65% loss of activity Helicobacter pylori
3.5.3.1 -20°C, purified recombinant enzyme, 1:2 with glycerol, loss of 45-65% activity after 4 months, and of over 90% after 6 months Helicobacter pylori
3.5.3.1 4°C, His-tagged recombinant protein, 1:2 dilution with 100% glycerol, 1 week, 90% loss of activity Helicobacter pylori
3.5.3.1 4°C, purified recombinant enzyme, 1:2 with glycerol, over 90% loss of activity within 1 week Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.1 L-arginine + H2O
-
 Helicobacter pylori L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O enzyme is involved in acid resistance and inhibits host nitric oxide production Helicobacter pylori L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O
-
 Helicobacter pylori ATCC 43504 L-ornithine + urea
-
 ?
3.5.3.1 L-arginine + H2O enzyme is involved in acid resistance and inhibits host nitric oxide production Helicobacter pylori ATCC 43504 L-ornithine + urea
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.3.1 ? x * 37800-40000, about, His-tagged enzyme, sequence calculation and SDS-PAGE Helicobacter pylori
3.5.3.1 ? x * 40000, SDS-PAGE, recombinant enzyme with His-tag Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
3.5.3.1 More enzyme belongs to the arginase superfamily Helicobacter pylori
3.5.3.1 RocF
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.1 additional information
-
 assay requires heat activation of enzyme in presence of metal cofactor at 50-55°C Helicobacter pylori
3.5.3.1 30
-
-
 Helicobacter pylori
3.5.3.1 37
-
 assay at Helicobacter pylori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.3.1 25 42 no reproducible temperature preference within this range Helicobacter pylori

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.1 50 55 activation in presence of Co2+ Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.1 6.1
-
-
 Helicobacter pylori
3.5.3.1 6.1
-
 recombinant enzyme Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.1 5.3 8.7 about 50% of amximal activity at pH 5.3 and pH 8.7 Helicobacter pylori