Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fritzsche, H.B.; Schwede, T.; Schulz, G.E.
    Covalent and three-dimensional structure of the cyclodextrinase from Flavobacterium sp. no. 92 (2003), Eur. J. Biochem., 270, 2332-2341.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 DNA and amino acid sequence determination and analysis, expression as native and selenomethionine derivatives in Eschrichia coli BL21(DE3) and B834 (DE3), respectively Flavobacterium sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.54 6 mg/ml purified recombinant enzyme as native and selenomethionine derivatives in 50 mM HEPES, pH 7.5, 3.8 M NaCl, and 3 mM DTT with the SeM-enzyme derivative, equal volumes of protein and reservoir solution, hanging drop vapour diffusion method, 2 days, 15% glycerol for cryoprotection, multiwavelength anomalous X-ray diffraction 3D-structure determination and analysis at 2.1 A resolution Flavobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.54 Ca2+ determination of the binding site Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Flavobacterium sp. Q8KKG0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 recombinant from Escherichia coli Flavobacterium sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.54 cyclomaltodextrin + H2O = linear maltodextrin active site structure Flavobacterium sp.
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclomaltodextrin + H2O
-
 Flavobacterium sp. alpha-D-glucose + maltose
-
 ?
3.2.1.54 beta-cyclomaltodextrin + H2O
-
 Flavobacterium sp. alpha-D-glucose + maltose
-
 ?
3.2.1.54 gamma-cyclomaltodextrin + H2O
-
 Flavobacterium sp. alpha-D-glucose + maltose
-
 ?
3.2.1.54 pullulan + H2O
-
 Flavobacterium sp. ?
-
 ?
3.2.1.54 starch + H2O
-
 Flavobacterium sp. alpha-D-glucose + maltose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 CD-degrading enzyme
-
 Flavobacterium sp.
3.2.1.54 CDase
-
 Flavobacterium sp.
3.2.1.54 cyclodextrinase
-
 Flavobacterium sp.
3.2.1.54 More enzyme belongs to the glycosylhydrolase family 13 Flavobacterium sp.