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Literature summary extracted from

  • Graciet, E.; Lebreton, S.; Camadro, J.M.; Gontero, B.
    Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase. Kinetic evidence for confromation changes upon association with the small protein CP12 (2003), Eur. J. Biochem., 270, 129-136.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.13 overexpression in Escherichia coli Chlamydomonas reinhardtii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.13 additional information
-
 additional information no Michaelis-Menten kinetics with 1,3-bisphosphoglycerate Chlamydomonas reinhardtii
1.2.1.13 0.018
-
 NADPH pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
1.2.1.13 0.028
-
 NADPH pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
1.2.1.13 0.12
-
 NADH pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
1.2.1.13 0.136
-
 NADH pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.13 36854
-
 4 * 36854, native A4 glyceraldehyde 3-phosphate dehydrogenase, MALDI-TOF Chlamydomonas reinhardtii
1.2.1.13 37072
-
 4 * 37072, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, MALDI-TOF Chlamydomonas reinhardtii
1.2.1.13 152000
-
 native A4 glyceraldehyde 3-phosphate dehydrogenase in complex with the small protein CP12, gel filtration Chlamydomonas reinhardtii
1.2.1.13 155000
-
 recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, gel filtration Chlamydomonas reinhardtii

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.13 Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.13
-
 Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.13 3-phospho-D-glyceroyl phosphate + NADH
-
 Chlamydomonas reinhardtii D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?
1.2.1.13 3-phospho-D-glyceroyl phosphate + NADPH
-
 Chlamydomonas reinhardtii D-glyceraldehyde 3-phosphate + phosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.13 tetramer 4 * 36854, native A4 glyceraldehyde 3-phosphate dehydrogenase, MALDI-TOF Chlamydomonas reinhardtii
1.2.1.13 tetramer 4 * 37072, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, MALDI-TOF Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
1.2.1.13 A4 glyceraldehyde 3-phosphate dehydrogenase
-
 Chlamydomonas reinhardtii
1.2.1.13 GAPDH
-
 Chlamydomonas reinhardtii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.13 40
-
 3-phospho-D-glyceroyl phosphate pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADH Chlamydomonas reinhardtii
1.2.1.13 41
-
 NADH pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
1.2.1.13 88
-
 3-phospho-D-glyceroyl phosphate pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADH Chlamydomonas reinhardtii
1.2.1.13 104
-
 NADH pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
1.2.1.13 223
-
 3-phospho-D-glyceroyl phosphate pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADPH Chlamydomonas reinhardtii
1.2.1.13 251
-
 NADPH pH 7.7, 30°C, native A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii
1.2.1.13 419
-
 3-phospho-D-glyceroyl phosphate pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase, reaction with NADPH Chlamydomonas reinhardtii
1.2.1.13 430
-
 NADPH pH 7.7, 30°C, recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.13 7.7
-
 native enzyme, reaction with NADPH Chlamydomonas reinhardtii
1.2.1.13 7.8
-
 native and recombinant enzyme, reaction with NADH Chlamydomonas reinhardtii
1.2.1.13 7.9
-
 recombinant enzyme, reaction with NADPH Chlamydomonas reinhardtii

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.13 NADH greater specificity for NADPH than for NADH Chlamydomonas reinhardtii
1.2.1.13 NADPH greater specificity for NADPH than for NADH Chlamydomonas reinhardtii