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Literature summary extracted from

  • Han, J.S.; Kosugi, Y.; Ishida, H.; Ishikawa, K.
    Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1 (2002), Eur. J. Biochem., 269, 969-976.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.261 expressed in Escherichia coli BL21(DE3) Aeropyrum pernix

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.261 K+ slight inhibition at 70 mM Aeropyrum pernix
1.1.1.261 Na+ slight inhibition at 70 mM Aeropyrum pernix
1.1.1.261 NAD(P)+ competitive with NAD(P)H and noncompetitive with dihydroxyacetone phosphate Aeropyrum pernix
1.1.1.261 sn-glycerol-1-phosphate noncompetitive with dihydroxyacetone phosphate and NAD(P)H Aeropyrum pernix

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.261 0.032
-
 NADH pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 0.044
-
 NADPH pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 0.29
-
 dihydroxyacetone phosphate in the presence of 0.32 mM NADPH, pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 0.46
-
 dihydroxyacetone phosphate in the presence of 0.32 mM NADH, pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 1.57
-
 NAD+ pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 8.92
-
 sn-glycerol-1-phosphate pH 7.0, 65°C Aeropyrum pernix

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.261 38000
-
 2 * 38000, SDS-PAGE Aeropyrum pernix
1.1.1.261 72400
-
 gel filtration Aeropyrum pernix

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.261 dihydroxyacetone phosphate + NAD(P)H Aeropyrum pernix key enzyme in the formation of archeal enantiomeric polar lipid structures sn-glycerol-1-phosphate + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.261 Aeropyrum pernix
-
 hyperthermophilic archeon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.261 recombinant protein Aeropyrum pernix

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.261 sn-glycerol 1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H + H+ ordered bi-bi mechanism Aeropyrum pernix
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.261 3.22
-
 purified enzyme, pH 7.0, 65°C Aeropyrum pernix

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.261 dihydroxyacetone phosphate + NAD(P)H key enzyme in the formation of archeal enantiomeric polar lipid structures Aeropyrum pernix sn-glycerol-1-phosphate + NAD(P)+
-
 r
1.1.1.261 dihydroxyacetone phosphate + NAD(P)H reverse reaction only with NAD+ as cofactor Aeropyrum pernix sn-glycerol-1-phosphate + NAD(P)+
-
 r
1.1.1.261 additional information no activity with glycerol-2-phosphate, glycerol-3-phosphate, glyceraldehyde phosphate, glycerol, and dihydroxyacetone Aeropyrum pernix ?
-
 ?
1.1.1.261 sn-glycerol-1-phosphate + NAD+ no reaction with NADP+ Aeropyrum pernix dihydroxyacetone phosphate + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.261 dimer 2 * 38000, SDS-PAGE Aeropyrum pernix

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.261 94 96
-
 Aeropyrum pernix

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.261 55 96
-
 Aeropyrum pernix

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.261 90
-
 half-life: 2 h Aeropyrum pernix
1.1.1.261 95
-
 half-life: 0.5 h Aeropyrum pernix
1.1.1.261 96
-
 unstable above 96°C Aeropyrum pernix

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.261 0.044
-
 NADPH pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 0.09
-
 NAD+ pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 0.09
-
 sn-glycerol-1-phosphate pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 0.75
-
 dihydroxyacetone phosphate in the presence of 0.32 mM NADPH, pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 2.4
-
 NADH pH 7.0, 65°C Aeropyrum pernix
1.1.1.261 2.6
-
 dihydroxyacetone phosphate in the presence of 0.32 mM NADH, pH 7.0, 65°C Aeropyrum pernix

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.261 7
-
-
 Aeropyrum pernix

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.261 NAD+
-
 Aeropyrum pernix
1.1.1.261 NADH
-
 Aeropyrum pernix
1.1.1.261 NADP+ no activity with sn-glycerol-1-phosphate as substrate Aeropyrum pernix
1.1.1.261 NADPH
-
 Aeropyrum pernix