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Literature summary extracted from

  • Lad, L.; Mewies, M.; Basran, J.; Scrutton, N.S.; Raven, E.L.
    Role of histidine 42 in ascorbate peroxidase. Kinetic analysis of the H42A and H42E variants (2002), Eur. J. Biochem., 269, 3182-3192.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.11.1.11 H42A inactive, partial recovery of activity by addition of exogenous imidazoles Pisum sativum
1.11.1.11 H42E decrease in kcat- and Km-value Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.17
-
 L-ascorbate pH 7.0, 25°C, mutant H42E Pisum sativum
1.11.1.11 0.41
-
 L-ascorbate pH 7.0, 25°C, wild-type Pisum sativum
1.11.1.11 2.9
-
 guaiacol pH 7.0, 25°C, mutant H42E Pisum sativum
1.11.1.11 12.3
-
 guaiacol pH 7.0, 25°C, wild-type Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.11 cytosol
-
 Pisum sativum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.11 Iron predominantly five-coordinate high-spin iron Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Pisum sativum
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.11.1.11 2 L-ascorbate + H2O2 + 2 H+ = 2 monodehydroascorbate + 2 H2O mechanism involves accumulation of a transient enzyme intermediate Pisum sativum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 guaiacol + H2O2
-
 Pisum sativum ? + H2O
-
 ?
1.11.1.11 L-ascorbate + H2O2
-
 Pisum sativum ? + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.11 1.5
-
 guaiacol pH 7.0, 25°C, mutant H42E Pisum sativum
1.11.1.11 3.9
-
 L-ascorbate pH 7.0, 25°C, mutant H42E Pisum sativum
1.11.1.11 66
-
 guaiacol pH 7.0, 25°C, wild-type Pisum sativum
1.11.1.11 248
-
 L-ascorbate pH 7.0, 25°C, wild-type Pisum sativum