BRENDA - Enzyme Database

Purification and partial characterization of barley glutamyl-tRNAGlu reductase,the enzyme that directs glutamate to chlorophyll biosynthesis

Pontoppida, B.; Kannangara, C.G.; Eur. J. Biochem. 225, 529-537 (1994)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
Cd2+
1 mM Cd(Ac)2, 92% inhibition
Hordeum vulgare
1.2.1.70
Co2+
10 mM CoCl2, 78% inhibition
Hordeum vulgare
1.2.1.70
Cu2+
1 mM CuCl2, 84% inhibition
Hordeum vulgare
1.2.1.70
Fe2+
5 mM FeSO4, 66% inhibition
Hordeum vulgare
1.2.1.70
Fe3+
10 mM FeCl3, 80% inhibition
Hordeum vulgare
1.2.1.70
glutamate 1-semialdehyde
0.2 mM, 50% inhibition
Hordeum vulgare
1.2.1.70
heme
0.004 mM, 50% inhibition
Hordeum vulgare
1.2.1.70
Zn2+
2.5 mM, ZnSO4, 94% inhibition
Hordeum vulgare
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.70
chloroplast
stroma of greening chloroplast
Hordeum vulgare
9507
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
54000
-
x * 54000, SDS-PAGE
Hordeum vulgare
1.2.1.70
270000
-
gel filtration
Hordeum vulgare
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Hordeum vulgare
the enzyme directs glutamate to chlorophyll biosynthesis
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Hordeum vulgare
-
cultivar Sval÷f Bonus
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.70
-
Hordeum vulgare
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.70
additional information
-
-
Hordeum vulgare
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
655401
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the enzyme directs glutamate to chlorophyll biosynthesis
655401
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.70
?
x * 54000, SDS-PAGE
Hordeum vulgare
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
Cd2+
1 mM Cd(Ac)2, 92% inhibition
Hordeum vulgare
1.2.1.70
Co2+
10 mM CoCl2, 78% inhibition
Hordeum vulgare
1.2.1.70
Cu2+
1 mM CuCl2, 84% inhibition
Hordeum vulgare
1.2.1.70
Fe2+
5 mM FeSO4, 66% inhibition
Hordeum vulgare
1.2.1.70
Fe3+
10 mM FeCl3, 80% inhibition
Hordeum vulgare
1.2.1.70
glutamate 1-semialdehyde
0.2 mM, 50% inhibition
Hordeum vulgare
1.2.1.70
heme
0.004 mM, 50% inhibition
Hordeum vulgare
1.2.1.70
Zn2+
2.5 mM, ZnSO4, 94% inhibition
Hordeum vulgare
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.70
chloroplast
stroma of greening chloroplast
Hordeum vulgare
9507
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
54000
-
x * 54000, SDS-PAGE
Hordeum vulgare
1.2.1.70
270000
-
gel filtration
Hordeum vulgare
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
Hordeum vulgare
the enzyme directs glutamate to chlorophyll biosynthesis
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
-
Hordeum vulgare
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.2.1.70
additional information
-
-
Hordeum vulgare
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
-
655401
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
the enzyme directs glutamate to chlorophyll biosynthesis
655401
Hordeum vulgare
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.70
?
x * 54000, SDS-PAGE
Hordeum vulgare