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Literature summary extracted from

  • Cepeljnik, T.; Krizaj, I.; Marinsek-Logar, R.
    Isolation and characterization of the Pseudobutyrivibrio xylanivorans Mz5T xylanase XynT - the first family 11 endoxylanase from rumen Butyrivibrio-related bacteria (2004), Enzyme Microb. Technol., 34, 219-227.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.8 nutrition possible usage as feed additive for animals in order to diminish health problems and enhance proliferation of beneficial microflora Pseudobutyrivibrio xylanivorans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.8 partial DNA and amino acid sequencing Pseudobutyrivibrio xylanivorans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 additional information
-
 separation of 2 isozymes with different MW by native PAGE Pseudobutyrivibrio xylanivorans
3.2.1.8 30000
-
 x * 30000, SDS-PAGE Pseudobutyrivibrio xylanivorans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Pseudobutyrivibrio xylanivorans
-
 rumen bacteria, strain Mz5T, DSM14809, enzyme XynT, 2 isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 by ammonium sulfate precipitation and hydrophobic interaction chromatography, to homogeneity, 2 isozymes Pseudobutyrivibrio xylanivorans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 14.5
-
 purified enzyme Pseudobutyrivibrio xylanivorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 additional information no activity against other polysaccharides, no arabinofuranosidase and beta-xylosidase activity, enzyme contains no xylan-binding domain Pseudobutyrivibrio xylanivorans ?
-
 ?
3.2.1.8 xylan + H2O substrate from brichwood, beechwood, or oat spelt soluble fraction, the latter being the best substrate, endoxylanase activity Pseudobutyrivibrio xylanivorans xylooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 30000, SDS-PAGE Pseudobutyrivibrio xylanivorans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 endoxylanase
-
 Pseudobutyrivibrio xylanivorans
3.2.1.8 More enzyme belongs to family 11 of glycosyl hydrolases Pseudobutyrivibrio xylanivorans
3.2.1.8 XynT
-
 Pseudobutyrivibrio xylanivorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 38
-
-
 Pseudobutyrivibrio xylanivorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 5.6
-
-
 Pseudobutyrivibrio xylanivorans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Pseudobutyrivibrio xylanivorans purified isozymes with 2 pI values, isoelectric focusing
-
 4.9
3.2.1.8 Pseudobutyrivibrio xylanivorans purified isozyme with 2 pI values, isoelectric focusing
-
 6