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Literature summary extracted from

  • Monti, D.; Baldaro, E.; Riva, S.
    Separation and characterization of two catalase activities isolated from the yeast Trigonopsis variabilis (2003), Enzyme Microb. Technol., 32, 596-605.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-Amino-1,2,4-triazole TvC-I and TvC-II, inactivation Trigonopsis variabilis
1.11.1.6 CN- TvC-I and TvC-II, at micromolar range Trigonopsis variabilis
1.11.1.6 additional information no reduction of TvC-II by dithionite Trigonopsis variabilis
1.11.1.6 NaN3 TvC-I and TvC-II, at micromolar range Trigonopsis variabilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.6 Fe2+ TvC-II, heme-containing Trigonopsis variabilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 62900
-
 4 * 62900, TvC-II, SDS-PAGE Trigonopsis variabilis
1.11.1.6 227300
-
 TvC-II, gel filtration Trigonopsis variabilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Trigonopsis variabilis detoxification enzyme O2 + H2O
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.11.1.6 chloroform ethanol/chloroform: TvC-I and TvC-II, quite stable to Trigonopsis variabilis
1.11.1.6 Ethanol ethanol/chloroform: TvC-I and TvC-II, quite stable to Trigonopsis variabilis

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Trigonopsis variabilis
-
 2 enzymes TvC-I and TvC-II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 TvC-II, 300fold to homogeneity, TvC-I partially purified, TvC-I separation from TvC-II Trigonopsis variabilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 117
-
 partially purified TvC-I Trigonopsis variabilis
1.11.1.6 55000
-
 purified TvC-II Trigonopsis variabilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Trigonopsis variabilis O2 + H2O
-
 ?
1.11.1.6 H2O2 detoxification enzyme Trigonopsis variabilis O2 + H2O
-
 ?
1.11.1.6 additional information 2 enzymes TvC-I and TvC-II do not show peroxidase ctivity Trigonopsis variabilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 tetramer 4 * 62900, TvC-II, SDS-PAGE Trigonopsis variabilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 25
-
 assay at Trigonopsis variabilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 55
-
 TvC-I and TvC-II, rapid inactivation Trigonopsis variabilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 8
-
 assay at Trigonopsis variabilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 3 10 pH-profile, TvC-I Trigonopsis variabilis
1.11.1.6 4 10 pH-profile, TvC-II Trigonopsis variabilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.11.1.6 1 2 TvC-I, half-life: at least 50 h Trigonopsis variabilis
1.11.1.6 1 3 TvC-I, half-life: about 2 h Trigonopsis variabilis
1.11.1.6 2
-
 and below, TvC-I is rapidly inactivated Trigonopsis variabilis
1.11.1.6 3
-
 TvC-I, half-life: at least 50 h Trigonopsis variabilis
1.11.1.6 4 10 TvC-II is rapidly inactivated below pH 4.0 and above pH 10.0, at pH 6.0-10.0 the half-life is approximately 20 h, at pH 5.0 it is 45 h, at pH 4.0 77 h Trigonopsis variabilis