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Literature summary extracted from

  • Thomas, J.L.; Mason, J.I.; Brandt, S.; Norris, W.
    Differences in substrate and inhibitor kinetics of human type 1 and type 2 3beta-hydroxysteroid dehydrogenase are explained by the type 1 mutant, H156Y (2002), Endocr. Res., 28, 471-475.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.145 expression in Sf9 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.145 H156Y 195% of wild-type activity Homo sapiens
1.1.1.145 K158Q no activity Homo sapiens
1.1.1.145 Y154F no activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.145 epostane
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.145 0.0037
-
 dehydroepiandrosterone pH 7.4 Homo sapiens
1.1.1.145 0.042
-
 dehydroepiandrosterone pH 7.4, H156Y mutant Homo sapiens
1.1.1.145 0.047
-
 dehydroepiandrosterone 27°C, pH 7.4 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+ Homo sapiens
-
 androst-5-en-3,17-dione + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.145 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.145 recombinant 3beta-HSD Homo sapiens
1.1.1.145 recombinant 3beta-HSD type 2 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.145 adrenal gland
-
 Homo sapiens
-
1.1.1.145 gonad
-
 Homo sapiens
-
1.1.1.145 mammary gland
-
 Homo sapiens
-
1.1.1.145 placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.145 dehydroepiandrosterone + NAD+
-
 Homo sapiens androst-5-en-3,17-dione + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.145 3beta-hydroxysteroid dehydrogenase type 1
-
 Homo sapiens
1.1.1.145 3beta-hydroxysteroid dehydrogenase type 2
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.145 0.000007
-
 epostane 27°C, pH 7.4 Homo sapiens
1.1.1.145 0.000098
-
 epostane 27°C, pH 7.4 Homo sapiens