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Literature summary extracted from

  • Bennett, M.J.; McKnight, S.L.; Coleman, J.P.
    Cloning and characterization of the NAD-dependent 7alpha-Hydroxysteroid dehydrogenase from Bacteroides fragilis (2003), Curr. Microbiol., 47, 475-484.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.159 expressed in Escherichia coli Bacteroides fragilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.159 HgCl2 complete inhibition at 7.5 mM Bacteroides fragilis
1.1.1.159 p-hydroxymercuribenzoate complete inhibition at 0.0075 mM Bacteroides fragilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.159 0.024
-
 chenodeoxycholic acid pH 8.5, 37°C Bacteroides fragilis
1.1.1.159 0.177
-
 cholic acid pH 8.5, 37°C Bacteroides fragilis
1.1.1.159 0.212
-
 NAD+ with cholic acid as substrate, pH 8.5, 37°C Bacteroides fragilis
1.1.1.159 0.709
-
 NAD+ with chenodeoxycholic acid as substrate, pH 8.5, 37°C Bacteroides fragilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.159 27400
-
 SDS-PAGE Bacteroides fragilis
1.1.1.159 110000
-
 gel filtration Bacteroides fragilis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Bacteroides fragilis Q9S3U5 strain ATCC 25285
-
1.1.1.159 Escherichia coli
-
-
-
1.1.1.159 Paeniclostridium sordellii
-
 strain ATCC 9714
-
1.1.1.159 [Clostridium] scindens
-
 formerly Eubacterium sp. strain VP1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159
-
 Bacteroides fragilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.159 351
-
 purified enzyme, 37°C Bacteroides fragilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 chenodeoxycholic acid + NAD+
-
 Escherichia coli 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 chenodeoxycholic acid + NAD+
-
 Paeniclostridium sordellii 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 chenodeoxycholic acid + NAD+
-
 [Clostridium] scindens 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 chenodeoxycholic acid + NAD+
-
 Bacteroides fragilis 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 cholic acid + NAD+
-
 Escherichia coli 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 cholic acid + NAD+
-
 Paeniclostridium sordellii 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 cholic acid + NAD+
-
 [Clostridium] scindens 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r
1.1.1.159 cholic acid + NAD+
-
 Bacteroides fragilis 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.159 tetramer 4 * 27400, subunit mass by SDS-PAGE, native mass by gel filtration Bacteroides fragilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.159 65
-
 complete loss of activity within 30 min Escherichia coli
1.1.1.159 65
-
 complete loss of activity within 30 min Paeniclostridium sordellii
1.1.1.159 65
-
 complete loss of activity within 30 min [Clostridium] scindens
1.1.1.159 65
-
 95% of initial activity after 1 h Bacteroides fragilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.159 6.5
-
 NADH oxidation Bacteroides fragilis
1.1.1.159 8.5
-
 NAD reduction Bacteroides fragilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.159 5.5 10.5 NAD reduction Bacteroides fragilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+
-
 Escherichia coli
1.1.1.159 NAD+
-
 Paeniclostridium sordellii
1.1.1.159 NAD+
-
 [Clostridium] scindens
1.1.1.159 NAD+
-
 Bacteroides fragilis
1.1.1.159 NADH
-
 Bacteroides fragilis