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Literature summary extracted from

  • Chauvatcharin, N.; Vattanaviboon, P.; Switala, J.; Loewen, P.C.; Mongkolsuk, S.
    Cloning and characterization of katA, encoding the major monofunctional catalase from Xanthomonas campestris pv. phaseoli and characterization of the encoded catalase KatA (2003), Curr. Microbiol., 46, 83-87.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.6 gene katA, screening of a genetic library, and DNA sequence determination and analysis, phylogenetic analysis, temperature-sensitive expression in enzyme-deficient strain Escherichia coli at 28°C, 200-300fold overexpression in a Xanthomonas campestris mutant strain XpHR Xanthomonas campestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 3-Amino-1,2,4-triazole strong inhibition Xanthomonas campestris
1.11.1.6 NaN3 strong inhibition Xanthomonas campestris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 75
-
 H2O2 pH 7.0, 37°C Xanthomonas campestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.6 H2O2 Xanthomonas campestris protective role of the enzyme O2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Xanthomonas campestris Q8L2Z7 pv. phaseoli, gene katA, enzyme KatA is the major enzyme of 2 different occurring in the organism
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 enzyme from a Xanthomonas campestris strain Xanthomonas campestris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 955
-
 recombinant enzyme Xanthomonas campestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2
-
 Xanthomonas campestris O2 + H2O
-
 ?
1.11.1.6 H2O2 protective role of the enzyme Xanthomonas campestris O2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.6 KatA
-
 Xanthomonas campestris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 37
-
 assay at Xanthomonas campestris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 30
-
 10 min, loss of 20% activity Xanthomonas campestris
1.11.1.6 55
-
 10 min, loss of 50% activity Xanthomonas campestris

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.6 255000
-
 H2O2 pH 7.0, 37°C Xanthomonas campestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 6 9 broad optimum Xanthomonas campestris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.6 6 9 rapid drop of activity below pH 6.0 and above pH 9.0 Xanthomonas campestris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.11.1.6 0.001
-
 NaN3 pH 7.0, 37°C Xanthomonas campestris
1.11.1.6 2
-
 3-Amino-1,2,4-triazole pH 7.0, 37°C Xanthomonas campestris