Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dmitryjuk, M.; Zoltowska, K.
    Purification and characterization of acid trehalase from muscle of Ascaris suum (Nematoda) (2003), Comp. Biochem. Physiol. B, 136, 61-69.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.28 EDTA 10 mM, 28% inhibition Ascaris suum
3.2.1.28 FeCl3 10 mM, 35% inhibition Ascaris suum
3.2.1.28 ZnSO4 10 mM, 34% inhibition Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.28 6.6
-
 alpha,alpha-trehalose
-
 Ascaris suum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.28 CaCl2 10 mM, activates 1.2fold Ascaris suum
3.2.1.28 iodoacetate 10 mM, activates 1.3fold Ascaris suum
3.2.1.28 KNO3 10 mM, activates 2.2fold Ascaris suum
3.2.1.28 MgCl2 10 mM, activates 2.4fold Ascaris suum
3.2.1.28 NaCl 10 mM, activates 1.4fold Ascaris suum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.28 38000
-
 2 * 38000, SDS-PAGE Ascaris suum
3.2.1.28 76000
-
 nondenaturing-PAGE Ascaris suum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.28 Ascaris suum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.28
-
 Ascaris suum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.28 muscle
-
 Ascaris suum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.28 0.531
-
-
 Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.28 alpha,alpha-trehalose + H2O
-
 Ascaris suum alpha-D-glucopyranose + D-glucose
-
 ?
3.2.1.28 isomaltose + H2O
-
 Ascaris suum alpha-D-glucopyranose
-
 ?
3.2.1.28 maltose + H2O
-
 Ascaris suum alpha-D-glucopyranose + D-glucose
-
 ?
3.2.1.28 melizitose + H2O weak Ascaris suum beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?
3.2.1.28 sucrose + H2O
-
 Ascaris suum beta-D-fructofuranose + alpha-D-glucopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.28 dimer 2 * 38000, SDS-PAGE Ascaris suum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.28 40
-
-
 Ascaris suum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.28 30 65 30°C: about 65% of maximal activity, 65°C: about 40% of maximal activity Ascaris suum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.28 50
-
 15 min, stable Ascaris suum
3.2.1.28 60
-
 15 min, 50% loss of activity Ascaris suum
3.2.1.28 65
-
 15 min, almost complete inactivation Ascaris suum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.28 4.9
-
-
 Ascaris suum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.28 2.6 7.9 pH 3.2: about 45% of maximal activity, pH 6.9: about 40% of maximal activity Ascaris suum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.28 Ascaris suum
-
-
 4.2