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Literature summary extracted from

  • Grossmann, G.A.; Terra, W.R.
    alpha-Galactosidases from the larval midgut of Tenebrio molitor (coleoptera) and Spodoptera frugiperda (lepidoptera) (2001), Comp. Biochem. Physiol. B, 128, 109-122.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 1-ethyl-3-(dimethylaminopropyl) carbodiimide inactivation by 1-ethyl-3-(dimethylaminopropyl) carbodiimide plus glycine ethyl ester follows a two-phase kinetics: a fast one decreases in presence of melibiose and another slow not affected by melibiose Spodoptera frugiperda
3.2.1.22 1-ethyl-3-(dimethylaminopropyl) carbodiimide inactivation by 1-ethyl-3-(dimethylaminopropyl) carbodiimide plus glycine ethyl ester follows a two-phase kinetics: a fast one decreases in presence of melibiose and another slow not affected by melibiose Tenebrio molitor
3.2.1.22 D-galactose
-
 Spodoptera frugiperda
3.2.1.22 D-galactose
-
 Tenebrio molitor
3.2.1.22 gamma-1,4-galactonolactone
-
 Spodoptera frugiperda
3.2.1.22 gamma-1,4-galactonolactone
-
 Tenebrio molitor
3.2.1.22 melibiose with p-nitrophenyl alpha-D-galactopyranoside as substrate Spodoptera frugiperda
3.2.1.22 melibiose with p-nitrophenyl alpha-D-galactopyranoside as substrate Tenebrio molitor
3.2.1.22 raffinose with p-nitrophenyl alpha-D-galactopyranoside as substrate Spodoptera frugiperda
3.2.1.22 raffinose with p-nitrophenyl alpha-D-galactopyranoside as substrate Tenebrio molitor
3.2.1.22 stachyose with p-nitrophenyl alpha-D-galactopyranoside as substrate Spodoptera frugiperda
3.2.1.22 stachyose with p-nitrophenyl alpha-D-galactopyranoside as substrate Tenebrio molitor
3.2.1.22 Tris
-
 Spodoptera frugiperda
3.2.1.22 Tris
-
 Tenebrio molitor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 1.7
-
 melibiose 30°C, pH 5.8 Spodoptera frugiperda
3.2.1.22 3.2
-
 p-nitrophenyl alpha-D-galactopyranoside 30°C, pH 5.0 Tenebrio molitor
3.2.1.22 3.8
-
 stachyose 30°C, pH 5.0 Tenebrio molitor
3.2.1.22 4.4
-
 raffinose 30°C, pH 5.0 Tenebrio molitor
3.2.1.22 4.8
-
 raffinose 30°C, pH 5.8 Spodoptera frugiperda
3.2.1.22 6.3
-
 melibiose 30°C, pH 5.0 Tenebrio molitor
3.2.1.22 7.2
-
 stachyose 30°C, pH 5.8 Spodoptera frugiperda
3.2.1.22 12
-
 p-nitrophenyl alpha-D-galactopyranoside 30°C, pH 5.8 Spodoptera frugiperda

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 46000
-
 2 * 46000 Spodoptera frugiperda
3.2.1.22 46000 49000 gel filtration Tenebrio molitor
3.2.1.22 94000
-
 ultracentrifugation Spodoptera frugiperda

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Spodoptera frugiperda
-
-
-
3.2.1.22 Tenebrio molitor
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 larva
-
 Tenebrio molitor
-
3.2.1.22 larva
-
 Spodoptera frugiperda
-
3.2.1.22 midgut of larva, three midgut alpha-galactosidases, SG1, SG2 and SG3 Spodoptera frugiperda
-
3.2.1.22 midgut of larva, three midgut alpha-galactosidases, TG1, TG2 and TG3 Tenebrio molitor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O 7.3% of Vmax with p-nitrophenyl alpha-D-galactopyranoside Tenebrio molitor D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O 35.5% of Vmax with p-nitrophenyl alpha-D-galactopyranoside Spodoptera frugiperda alpha-D-galactose + D-glucose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Tenebrio molitor p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Spodoptera frugiperda p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O 20% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside Tenebrio molitor sucrose + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O Vmax is 1.8fold higher than that with p-nitrophenyl alpha-D-galactopyranoside Spodoptera frugiperda sucrose + alpha-D-galactose
-
 ?
3.2.1.22 stachyose + 2 H2O 17.8% of the Vmax with p-nitrophenyl alpha-D-galactopyranoside Tenebrio molitor 2 alpha-D-galactose + sucrose
-
 ?
3.2.1.22 stachyose + 2 H2O Vmax is 2fold higher than that with p-nitrophenyl alpha-D-galactopyranoside Spodoptera frugiperda 2 alpha-D-galactose + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 dimer 2 * 46000 Spodoptera frugiperda

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5
-
-
 Tenebrio molitor
3.2.1.22 5.8
-
-
 Spodoptera frugiperda

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 4 6.5 pH 4.0: about 45% of maximal activity, pH 6.5: about 40% of maximal activity Tenebrio molitor
3.2.1.22 4.5 7.5 pH 4.5: about 40% of maximal activity, pH 7.5: about 40% of maximal activity Spodoptera frugiperda

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 3
-
 30°C, 2 h, about 50% loss of activity Tenebrio molitor
3.2.1.22 4 6 30°C, 2 h, stable Tenebrio molitor
3.2.1.22 5 7 stable Spodoptera frugiperda
3.2.1.22 7.5
-
 30°C, 2 h, about 20% loss of activity Tenebrio molitor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.22 3.5
-
 D-galactose 30°C, pH 5.0 Tenebrio molitor
3.2.1.22 5.6
-
 D-galactose 30°C, pH 5.8 Spodoptera frugiperda
3.2.1.22 6.6
-
 stachyose 30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate Spodoptera frugiperda
3.2.1.22 7.7
-
 melibiose 30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate Tenebrio molitor
3.2.1.22 9
-
 raffinose 30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate Spodoptera frugiperda
3.2.1.22 9.2
-
 gamma-1,4-galactonolactone 30°C, pH 5.0 Tenebrio molitor
3.2.1.22 9.6
-
 Tris 30°C, pH 5.0 Tenebrio molitor
3.2.1.22 10.3
-
 melibiose 30°C, pH 5.8, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate Spodoptera frugiperda
3.2.1.22 11.6
-
 stachyose 30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate Tenebrio molitor
3.2.1.22 12
-
 Tris 30°C, pH 5.8 Spodoptera frugiperda
3.2.1.22 13.1
-
 raffinose 30°C, pH 5.0, reaction with with p-nitrophenyl alpha-D-galactopyranoside as substrate Tenebrio molitor
3.2.1.22 43.1
-
 gamma-1,4-galactonolactone 30°C, pH 5.8 Spodoptera frugiperda

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.22 Tenebrio molitor isoelectric focusing
-
 4.6
3.2.1.22 Tenebrio molitor isoelectric focusing
-
 4.9
3.2.1.22 Spodoptera frugiperda isoelectric focusing
-
 7.1
3.2.1.22 Spodoptera frugiperda isoelectric focusing
-
 7.2