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Literature summary extracted from

  • Mohamed, S.A.; Christensen, T.M.I.E.; Mikkelsen, J.D.
    New polygalacturonases from Trichoderma reesei: characterization and their specificities to partially methylated and acetylated pectins (2003), Carbohydr. Res., 338, 515-524.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 63000
-
 PG2, gel filtration Trichoderma reesei
3.2.1.15 66000
-
 PG1, gel filtration Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Trichoderma reesei
-
 ATCC 26920
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15
-
 Trichoderma reesei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.15 culture filtrate
-
 Trichoderma reesei
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 additional information
-
-
 Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 pectin + H2O the two enzymes PG1 and PG2 have higher affinity towards polygalacturonate derived from sugar beet pectin than polygalacturonate derived from lime pectin. Enzymatic activity decreases with increased degree of methylation of lime pectins Trichoderma reesei oligogalacturonides
-
 ?
3.2.1.15 polygalacturonate + H2O the two enzymes PG1 and PG2 have higher affinity towards polygalacturonate derived from sugar beet pectin than polygalacturonate derived from lime pectin Trichoderma reesei oligogalacturonides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 monomer 1 * 66000, PG1 or PG2, SDS-PAGE Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 PG1
-
 Trichoderma reesei
3.2.1.15 PG2
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 40
-
 PG1 Trichoderma reesei
3.2.1.15 50
-
 PG2 Trichoderma reesei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.15 20 50 20°C: about 60% of maximal activity, 50°C: about 90% of maximal activity, 60°C: about 10% of maximal activity, PG1 Trichoderma reesei
3.2.1.15 30 60 30°C: about 60% of maximal activity, 60°C: about 75% of maximal activity, 70°C: about 25% of maximal activity, PG2 Trichoderma reesei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 40
-
 15 min, PG1 loses about 30% of its activity, PG2 loses about 10% of its activity Trichoderma reesei
3.2.1.15 50
-
 15 min, PG1 loses about 80% of its activity, PG2 loses about 10% of its activity Trichoderma reesei
3.2.1.15 60
-
 15 min, PG1 loses about 90% of its activity, PG2 loses about 20% of its activity Trichoderma reesei
3.2.1.15 70
-
 15 min, PG1 loses about 95% of its activity, PG2 loses about 85% of its activity Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 4.2
-
 PG2 Trichoderma reesei
3.2.1.15 4.5
-
 PG1 Trichoderma reesei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 3.8 4.5 pH 3.8: about 80% of maximal activity, pH 4.5: about 50% of maximal activity, PG2 Trichoderma reesei
3.2.1.15 3.8 4.9 pH 3.8: about 40% of maximal acticity, pH 4.9: about 50% of maximal activity, PG1 Trichoderma reesei