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Literature summary extracted from
- Characterization of cytosolic sialidase from Chinese hamster ovary cells: part I: cloning and expression of soluble sialidase in Escherichia coli (2001), Carbohydr. Res., 330, 335-346.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.18 | expression as glutathione-S-transferase fusion protein in Escherichia coli | Cricetulus griseus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.18 | Neu5Ac | i.e. alpha-D-N-acetylneuraminic acid, competitive, 17% inhibition at 2 mM, 20% inhibition at 5 mM with substrate 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | Cricetulus griseus |  |
| 3.2.1.18 | Neu5Ac2en | i.e. 2-deoxy-2,3-dehydro-alpha-D-N-acetylneuraminic acid, competitive, 49% inhibition at 0.1 mM, 75% inhibition at 0.5 mM with substrate 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid | Cricetulus griseus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.18 | cytosol | - |
Cricetulus griseus | 5829 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.18 | 43000 | - |
x * 69000, recombinant fusion enzyme, SDS-PAGE, x * 43000, untagged enzyme, SDS-PAGE | Cricetulus griseus |
| 3.2.1.18 | 69000 | - |
x * 69000, recombinant fusion enzyme, SDS-PAGE, x * 43000, untagged enzyme, SDS-PAGE | Cricetulus griseus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.18 | Cricetulus griseus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.18 | recombinant glutathione-S-transferase fusion protein from Escherichia coli, by a one-step glutathione-affinity chromatography, 40fold | Cricetulus griseus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.18 | CHO cell | - |
Cricetulus griseus | - |
| 3.2.1.18 | ovary | - |
Cricetulus griseus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.18 | 0.999 | - |
purified recombinant enzyme | Cricetulus griseus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.18 | 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O | - |
Cricetulus griseus | 4-methylumbelliferol + alpha-D-N-acetylneuraminic acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.18 | ? | x * 69000, recombinant fusion enzyme, SDS-PAGE, x * 43000, untagged enzyme, SDS-PAGE | Cricetulus griseus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.18 | sialidase | - |
Cricetulus griseus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.18 | 37 | - |
assay at | Cricetulus griseus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.18 | 5.5 | - |
assay at | Cricetulus griseus |
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