Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Taylor, G.; Jabaji-Hare, S.; Charest, P.M.; Khan, W.
    Purification and characterization of an extracellular exochitinase, beta-N-acetylhexosaminidase, from the fungal mycoparasite Stachybotrys elegans (2002), Can. J. Microbiol., 48, 311-319.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 CaCl2 1 mM, 13% inhibition Stachybotrys elegans
3.2.1.52 CoCl2 1 mM, 15% inhibition Stachybotrys elegans
3.2.1.52 MgCl2 1 mM, 13% inhibition Stachybotrys elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.0846
-
 p-nitrophenyl-N-acetyl-beta-D-glucosaminide
-
 Stachybotrys elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 68000
-
 2 * 68000, SDS-PAGE Stachybotrys elegans
3.2.1.52 120000
-
 gel filtration Stachybotrys elegans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.52 additional information Stachybotrys elegans the enzyme is induced when Stachybotrys elegans is grown on purified cell wall fragments of its host, Rhizoctonia solani, as well as during antagonistic interaction of the mycoparasite and host when both are grown on synthetic medium with or without supplemental carbon source ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Stachybotrys elegans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52
-
 Stachybotrys elegans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 14.27
-
-
 Stachybotrys elegans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 additional information the enzyme is induced when Stachybotrys elegans is grown on purified cell wall fragments of its host, Rhizoctonia solani, as well as during antagonistic interaction of the mycoparasite and host when both are grown on synthetic medium with or without supplemental carbon source Stachybotrys elegans ?
-
 ?
3.2.1.52 p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Stachybotrys elegans p-nitrophenol + N-acetyl-beta-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 dimer 2 * 68000, SDS-PAGE Stachybotrys elegans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 exo-acting chitinase
-
 Stachybotrys elegans
3.2.1.52 NAG-68
-
 Stachybotrys elegans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 40
-
-
 Stachybotrys elegans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 35 45 35°C: about 50% of maximal activity, 45°C: about 80% of maximal activity Stachybotrys elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5
-
-
 Stachybotrys elegans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 4 7 pH 4.0: about 80% of maximal activity, pH 7.0: about 50% of maximal activity Stachybotrys elegans