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Literature summary extracted from

  • Saha, B.C.
    Purification and characterization of a novel mannitol dehydrogenase from Lactobacillus intermedius (2004), Biotechnol. Prog., 20, 537-542.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.138 EDTA 10 mM, 60% inhibition Lactobacillus intermedius
1.1.1.138 additional information not inhibitory: CaCl2, MgCl2, Cocl2, dithiothreitol Lactobacillus intermedius
1.1.1.138 p-chloromercuribenzoate 0.2 mM, complete inhibition Lactobacillus intermedius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.138 20
-
 D-fructose pH 5.5, 30°C Lactobacillus intermedius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.138 34500
-
 2 * 43000 + 2 * 34500, SDS-PAGE, both subunits have the same amino-terminal amino acid sequence Lactobacillus intermedius
1.1.1.138 43000
-
 2 * 43000 + 2 * 34500, SDS-PAGE, both subunits have the same amino-terminal amino acid sequence Lactobacillus intermedius
1.1.1.138 170000
-
 gel filtration Lactobacillus intermedius

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.138 Lactobacillus intermedius
-
 NRRL B-3693, enzyme production depends on growth on fructose or sucrose
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.138
-
 Lactobacillus intermedius

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.138 331
-
 pH 5.5, 30°C Lactobacillus intermedius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.138 D-fructose + NADPH
-
 Lactobacillus intermedius D-mannitol + NADP+
-
 r
1.1.1.138 D-mannitol + NAD+ less than 25% of the activity with NADP+ Lactobacillus intermedius D-fructose + NADH + H+
-
 r
1.1.1.138 D-mannitol + NADP+
-
 Lactobacillus intermedius D-fructose + NADPH + H+
-
 r
1.1.1.138 additional information no substrate: glucose, xylose, arabinose Lactobacillus intermedius ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.138 heterotetramer 2 * 43000 + 2 * 34500, SDS-PAGE, both subunits have the same amino-terminal amino acid sequence Lactobacillus intermedius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.138 35
-
 reduction of D-fructose Lactobacillus intermedius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.138 50
-
 reduction of D-fructose, 44% of maximum activity Lactobacillus intermedius
1.1.1.138 60
-
 reduction of D-fructose, 15% of maximum activity Lactobacillus intermedius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.138 35
-
 stable up to Lactobacillus intermedius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.138 5.5
-
 reduction of D-fructose Lactobacillus intermedius
1.1.1.138 7
-
 oxidation of D-fructose Lactobacillus intermedius

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.138 5
-
 reduction of D-fructose, 35% of maximum activity Lactobacillus intermedius
1.1.1.138 6.5
-
 reduction of D-fructose, 50% of maximum activity Lactobacillus intermedius

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.138 4.5 8
-
 Lactobacillus intermedius

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.138 NADP+
-
 Lactobacillus intermedius
1.1.1.138 NADPH
-
 Lactobacillus intermedius

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.138 Lactobacillus intermedius isoelectric focusing
-
 4.9