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Literature summary extracted from

  • Kim, C.S.; Ji, E.S.; Oh, D.K.
    Expression and characterization of Kluyveromyces lactis beta-galactosidase in Escherichia coli (2003), Biotechnol. Lett., 25, 1769-1774.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 expression in Escherichia coli as a soluble His-tagged recombinant enzyme Kluyveromyces lactis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ba2+ 1 mM, 56% inhibition of activity with o-nitrophenyl beta-D-galactopyranoside Kluyveromyces lactis
3.2.1.23 Ca2+ 1 mM, 60% inhibition of activity with lactose Kluyveromyces lactis
3.2.1.23 Cu2+ 1 mM, 97% inhibition of activity with o-nitrophenyl beta-D-galactopyranoside, 88% inhibition of activity with lactose Kluyveromyces lactis
3.2.1.23 imidazole above 50 mM, irreversible Kluyveromyces lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 1.5
-
 o-nitrophenyl beta-D-galactopyranoside pH 7.0, 37°C Kluyveromyces lactis
3.2.1.23 19.8
-
 lactose pH 7.0, 37°C Kluyveromyces lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Ca2+ 1 mM, 1.85fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside Kluyveromyces lactis
3.2.1.23 Co2+ 1 mM, 8.1fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside, 4.7fold stimulation of activity with lactose Kluyveromyces lactis
3.2.1.23 Fe2+ 1 mM, 4.5fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside, 4.1fold stimulation of activity with lactose Kluyveromyces lactis
3.2.1.23 Mg2+ 1 mM, 7fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside, 5.4fold stimulation of activity with lactose Kluyveromyces lactis
3.2.1.23 Mn2+ 1 mM, 8.9fold stimulation of activity with o-nitrophenyl beta-D-galactopyranoside, 5.6fold stimulation of activity with lactose Kluyveromyces lactis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Kluyveromyces lactis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 lactose + H2O
-
 Kluyveromyces lactis D-glucose + D-galactose
-
 ?
3.2.1.23 o-nitrophenyl beta-D-galactopyranoside + H2O
-
 Kluyveromyces lactis o-nitrophenol + beta-D-galactose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 37
-
 hydrolysis of o-nitrophenyl beta-D-galactopyranoside Kluyveromyces lactis
3.2.1.23 40
-
 hydrolysis of lactose Kluyveromyces lactis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7
-
 hydrolysis of o-nitrophenyl beta-D-galactopyranoside or lactose Kluyveromyces lactis