EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.8 | paper production | use in treatment of paper pulps | Thermomyces lanuginosus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.8 | additional information | - |
additional information | - |
Thermomyces lanuginosus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.2.1.8 | extracellular | - |
Thermomyces lanuginosus | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 22000 | - |
wild-type enzyme, gel filtration | Thermomyces lanuginosus |
3.2.1.8 | 24000 | - |
mutant enzyme, gel filtration | Thermomyces lanuginosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.8 | Thermomyces lanuginosus | - |
strain ATCC 44008, cellulase-free wild-type R-MB and mutant M1 | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.2.1.8 | glycoprotein | 0.74% carbohydrate content of the wild-type enzyme, 11.8% of the mutant enzyme | Thermomyces lanuginosus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.8 | native wild-type, 17fold, and mutant enzymes, to homogeneity by anion exchange chromatography and gel filtration | Thermomyces lanuginosus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.8 | (Xylbeta(1-4))n + H2O = (Xylbeta(1-4))n-m + (Xylbeta(1-4))m | histidine, tryptophan residues and carboxylic groups are involved in substrate binding or catalysis | Thermomyces lanuginosus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.2.1.8 | additional information | organism is grown on corn cobs | Thermomyces lanuginosus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 1660 | - |
purified wild-type enzyme | Thermomyces lanuginosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.8 | 4-O-methyl-glucuronoxylan + H2O | - |
Thermomyces lanuginosus | ? | - |
? | |
3.2.1.8 | additional information | no activity with mannan, araban, galactomannan, inulin, Avicel, soluble starch, sodium carboxymethylcellulose, 2- or 4-nitrophenyl-gluco-, xylo-, manno-, galacto-pyranosides, and arabinofuranoside | Thermomyces lanuginosus | ? | - |
? | |
3.2.1.8 | xylan + H2O | commercial substrate and from oat spelts and larch sawdust, the latter being the best substrate for wild-type and mutant enzyme | Thermomyces lanuginosus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.8 | monomer | 1 * 22000, wild-type enzyme, SDS-PAGE, 1 * 24000, mutant enzyme, SDS-PAGE | Thermomyces lanuginosus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.8 | More | the enzyme belongs to family 11 of glycosyl hydrolases | Thermomyces lanuginosus |
3.2.1.8 | xylanase | - |
Thermomyces lanuginosus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 60 | - |
wild-type enzyme | Thermomyces lanuginosus |
3.2.1.8 | 70 | - |
mutant enzyme | Thermomyces lanuginosus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 70 | - |
mutant enzyme retains over 50% activity after 2 h | Thermomyces lanuginosus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 6.7 | - |
mutant enzyme | Thermomyces lanuginosus |
3.2.1.8 | 7 | - |
wild-type enzyme | Thermomyces lanuginosus |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 3 | 9 | - |
Thermomyces lanuginosus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.8 | 8.3 | - |
wild-type and mutant enzymes, stable up to 24 h at 50°C, 60% remaining activity | Thermomyces lanuginosus |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.8 | Thermomyces lanuginosus | mutant enzyme | - |
6 |
3.2.1.8 | Thermomyces lanuginosus | wild-type enzyme | - |
6 |