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Literature summary extracted from

  • Xiao, M.; Tanaka, K.; Quian, X.M.; Yamamoto, K.; Kumagai, H.
    High-yield production and characterization of alpha-galactosidase from Bifidobacterium breve grown on raffinose (2000), Biotechnol. Lett., 22, 747-751.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 Cu2+ 1 mM, strong inhibition Bifidobacterium breve
3.2.1.22 Hg2+ 1 mM, strong inhibition Bifidobacterium breve

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.66
-
 raffinose pH 5.5, 37°C Bifidobacterium breve
3.2.1.22 2.14
-
 melibiose pH 5.5, 37°C Bifidobacterium breve

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.22 intracellular
-
 Bifidobacterium breve 5622
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 80000
-
 2 * 80000, SDS-PAGE Bifidobacterium breve
3.2.1.22 160000
-
 non-denaturing PAGE Bifidobacterium breve

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.22 additional information Bifidobacterium breve raffinose induces about twice the activity than melibiose ?
-
 ?
3.2.1.22 additional information Bifidobacterium breve 203 raffinose induces about twice the activity than melibiose ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Bifidobacterium breve
-
-
-
3.2.1.22 Bifidobacterium breve 203
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Bifidobacterium breve

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.22 culture condition:raffinose-grown cell
-
 Bifidobacterium breve
-
3.2.1.22 additional information cell grown on raffinose Bifidobacterium breve
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 215
-
-
 Bifidobacterium breve

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O
-
 Bifidobacterium breve alpha-D-galactose + D-glucose
-
 ?
3.2.1.22 melibiose + H2O
-
 Bifidobacterium breve 203 alpha-D-galactose + D-glucose
-
 ?
3.2.1.22 additional information raffinose induces about twice the activity than melibiose Bifidobacterium breve ?
-
 ?
3.2.1.22 additional information raffinose induces about twice the activity than melibiose Bifidobacterium breve 203 ?
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Bifidobacterium breve p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Bifidobacterium breve 203 p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Bifidobacterium breve sucrose + alpha-D-galactose
-
 ?
3.2.1.22 raffinose + H2O
-
 Bifidobacterium breve 203 sucrose + alpha-D-galactose
-
 ?
3.2.1.22 stachyose + 2 H2O
-
 Bifidobacterium breve 2 alpha-D-galactose + sucrose
-
 ?
3.2.1.22 stachyose + 2 H2O
-
 Bifidobacterium breve 203 2 alpha-D-galactose + sucrose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 dimer 2 * 80000, SDS-PAGE Bifidobacterium breve

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5.5 6.5
-
 Bifidobacterium breve

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 5.5 12 4°C, overnight, stable Bifidobacterium breve