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Literature summary extracted from

  • Nakajima, Y.; Kobayashi, K.; Yamagishi, K.; Enomoto, T.; Ohmiya, Y.
    cDNA cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca (2004), Biosci. Biotechnol. Biochem., 68, 565-570.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.13.12.6 biotechnology use of enzyme as a potent secreted reporter Cypridina noctiluca

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.12.6 0.03
-
 1-{3-[6-(1H-indol-3-yl)-2-(1-methylpropyl)-3-oxo-3,7-dihydroimidazo[1,2-a]pyrazin-8-yl]propyl}guanidine pH 7.4 Cypridina noctiluca

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.12.6 61415
-
 x * 61415, deduced from gene sequence Cypridina noctiluca

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.6 Cypridina noctiluca Q75R40
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.12.6 culture supernatant
-
 Cypridina noctiluca
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.6 1-{3-[6-(1H-indol-3-yl)-2-(1-methylpropyl)-3-oxo-3,7-dihydroimidazo[1,2-a]pyrazin-8-yl]propyl}guanidine + O2
-
 Cypridina noctiluca N-[3-(3-carbamimidamidopropyl)-5-(1H-indol-3-yl)-1,4-dihydropyrazin-2-yl]-2-methylbutanamide + CO2 + hn
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.12.6 ? x * 61415, deduced from gene sequence Cypridina noctiluca

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.12.6 37
-
 53 h, 50% residual activity Cypridina noctiluca

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.12.6 Cypridina noctiluca calculated
-
 4.9