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Literature summary extracted from

  • Fujita, K.; Takami, H.; Yamamoto, K.; Takegawa, K.
    Characterization of endo-beta-N-acetylglucosaminidase from alkaliphilic Bacillus halodurans C-125 (2004), Biosci. Biotechnol. Biochem., 68, 1059-1066.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.96 expressed in Escherichia coli Halalkalibacterium halodurans

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.96 E172Q mutant has no enzymatic activity Halalkalibacterium halodurans
3.2.1.96 additional information deletion of 219 C-terminal amino acid residues, deletion mutant shows wild-type activity Halalkalibacterium halodurans
3.2.1.96 W215R enzyme has lower transglycosylation activity Halalkalibacterium halodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.96 90000
-
 SDS-PAGE Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.96 Halalkalibacterium halodurans
-
-
-
3.2.1.96 Halalkalibacterium halodurans C-125
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.96 partial Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.96 Asn-(GlcNAc)2(Man)6 + gentiobiose enzyme shows transglycosylation activity in the presence of glucose and gentiobiose, but with galactose Halalkalibacterium halodurans ?
-
 ?
3.2.1.96 Asn-(GlcNAc)2(Man)6 + gentiobiose enzyme shows transglycosylation activity in the presence of glucose and gentiobiose, but with galactose Halalkalibacterium halodurans C-125 ?
-
 ?
3.2.1.96 Asn-(GlcNAc)2(Man)6 + glucose enzyme shows transglycosylation activity in the presence of glucose and gentiobiose, but with galactose Halalkalibacterium halodurans ?
-
 ?
3.2.1.96 Asn-(GlcNAc)2(Man)6 + glucose enzyme shows transglycosylation activity in the presence of glucose and gentiobiose, but with galactose Halalkalibacterium halodurans C-125 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.96 Endo-BH
-
 Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.96 6 7
-
 Halalkalibacterium halodurans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.96 9
-
 60% activity at pH 9 Halalkalibacterium halodurans
3.2.1.96 10
-
 28% activity at pH 10 Halalkalibacterium halodurans